Faculty of Chemistry, Jagiellonian University, 3 Ingardena Str., 30-060 Krakow, Poland.
Analyst. 2013 Nov 7;138(21):6645-52. doi: 10.1039/c3an01050c.
In this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1) range allowed for quantitative analysis of the atherosclerosis plaque area, the results of which were highly correlated with those of Oil Red O histological staining (R(2) = 0.935). Moreover, a specific class obtained from a second cluster analysis of the aortic cross-section samples at different stages of disease progression (3, 4 and 6 months old) seemed to represent the macrophages (CD68) area within the atherosclerotic plaque.
本工作通过傅里叶变换红外成像（FT-IR 成像）定量测定动脉粥样硬化病变面积（ApoE/LDLR(-/-) 小鼠），并通过与经典油红 O 染色法测定的动脉粥样硬化病变面积进行比较进行验证。在 2800-3025 cm(-1) 范围内基于 FT-IR 的测量的聚类分析允许对动脉粥样硬化斑块面积进行定量分析，其结果与油红 O 组织学染色高度相关（R(2) = 0.935）。此外，从疾病进展不同阶段（3、4 和 6 个月大）的主动脉横截面样本的第二次聚类分析中获得的一个特定类别似乎代表了动脉粥样硬化斑块内的巨噬细胞（CD68）区域。
