牙龈卟啉单胞菌蛋白酶寡聚体向非共价细胞表面附着复合物的解聚。
Disruption of gingipain oligomerization into non-covalent cell-surface attached complexes.
机构信息
Oral Health and Systemic Diseases Research Group, University of Louisville School of Dentistry, Louisville, KY 40202, USA.
出版信息
Biol Chem. 2012 Sep;393(9):971-7. doi: 10.1515/hsz-2012-0175.
Abstract
RgpA and Kgp gingipains are non-covalent complexes of endoprotease catalytic and hemagglutinin-adhesin domains on the surface of Porphyromonas gingivalis. A motif conserved in each domain has been suggested to function as an oligomerization motif. We tested this hypothesis by mutating motif residues to hexahistidine or insertion of hexahistidine tag to disrupt the motif within the Kgp catalytic domain. All modifications led to the secretion of entire Kgp activity into the growth media, predominantly in a form without functional His-tag. This confirmed the role of the conserved motif in correct posttranslational proteolytic processing and assembly of the multidomain complexes.
摘要
RgpA 和 Kgp 牙龈蛋白酶是牙龈卟啉单胞菌表面内切蛋白酶催化结构域和血凝素-黏附结构域的非共价复合物。每个结构域中都有一个保守的基序，被认为是一个寡聚基序。我们通过将 motif 残基突变为六组氨酸或插入六组氨酸标签来破坏 Kgp 催化结构域中的 motif，从而验证了这一假设。所有的修饰都导致了完整的 Kgp 活性分泌到生长培养基中，主要是以没有功能 His 标签的形式。这证实了保守基序在正确的翻译后蛋白水解加工和多结构域复合物组装中的作用。
相似文献
1
Disruption of gingipain oligomerization into non-covalent cell-surface attached complexes.牙龈卟啉单胞菌蛋白酶寡聚体向非共价细胞表面附着复合物的解聚。
Biol Chem. 2012 Sep;393(9):971-7. doi: 10.1515/hsz-2012-0175.
2
Porphyromonas gingivalis gingipains: the molecular teeth of a microbial vampire.牙龈卟啉单胞菌牙龈蛋白酶：微生物“吸血鬼”的分子牙齿
Curr Protein Pept Sci. 2003 Dec;4(6):409-26. doi: 10.2174/1389203033487009.
3
Structural insights unravel the zymogenic mechanism of the virulence factor gingipain K from , a causative agent of gum disease from the human oral microbiome.结构解析揭示了牙龈蛋白酶K（一种来自人类口腔微生物群的牙龈疾病病原体）的毒力因子的酶原激活机制。
J Biol Chem. 2017 Apr 7;292(14):5724-5735. doi: 10.1074/jbc.M117.776724. Epub 2017 Feb 14.
4
The RgpB C-terminal domain has a role in attachment of RgpB to the outer membrane and belongs to a novel C-terminal-domain family found in Porphyromonas gingivalis.RgpB的C末端结构域在RgpB附着于外膜的过程中发挥作用，并且属于在牙龈卟啉单胞菌中发现的一个新型C末端结构域家族。
J Bacteriol. 2006 Sep;188(17):6376-86. doi: 10.1128/JB.00731-06.
5
Molecular cloning and characterization of Porphyromonas gingivalis lysine-specific gingipain. A new member of an emerging family of pathogenic bacterial cysteine proteinases.牙龈卟啉单胞菌赖氨酸特异性牙龈蛋白酶的分子克隆与特性分析。一种新出现的致病性细菌半胱氨酸蛋白酶家族的新成员。
J Biol Chem. 1997 Jan 17;272(3):1595-600. doi: 10.1074/jbc.272.3.1595.
6
RgpA-Kgp peptide-based immunogens provide protection against Porphyromonas gingivalis challenge in a murine lesion model.基于RgpA-Kgp肽的免疫原在小鼠损伤模型中对牙龈卟啉单胞菌攻击具有保护作用。
Infect Immun. 2000 Jul;68(7):4055-63. doi: 10.1128/IAI.68.7.4055-4063.2000.
7
The K1K2 region of Lys-gingipain of Porphyromonas gingivalis blocks induction of HLA expression by gamma interferon.牙龈卟啉单胞菌赖氨酰-精氨酸蛋白酶的 K1K2 区阻断γ干扰素诱导 HLA 表达。
Infect Immun. 2012 Oct;80(10):3733-41. doi: 10.1128/IAI.00528-12. Epub 2012 Jul 16.
8
Propeptide-mediated inhibition of cognate gingipain proteinases.原肽介导的同源牙龈蛋白酶的抑制作用。
PLoS One. 2013 Jun 10;8(6):e65447. doi: 10.1371/journal.pone.0065447. Print 2013.
9
Structure and mechanism of cysteine peptidase gingipain K (Kgp), a major virulence factor of Porphyromonas gingivalis in periodontitis.半胱氨酸蛋白酶牙龈蛋白酶K（Kgp）的结构与机制，牙龈卟啉单胞菌在牙周炎中的一种主要毒力因子
J Biol Chem. 2014 Nov 14;289(46):32291-32302. doi: 10.1074/jbc.M114.602052. Epub 2014 Sep 29.
10
Structure of the lysine specific protease Kgp from Porphyromonas gingivalis, a target for improved oral health.牙龈卟啉单胞菌赖氨酸特异性蛋白酶Kgp的结构，改善口腔健康的一个靶点。
Protein Sci. 2015 Jan;24(1):162-6. doi: 10.1002/pro.2589. Epub 2014 Dec 3.
引用本文的文献
1
Discovery of Gingipains and Inhibitors from Food-Derived Natural Products: A Narrative Review.从食物来源的天然产物中发现牙龈蛋白酶及其抑制剂：一篇综述
Foods. 2025 Aug 19;14(16):2869. doi: 10.3390/foods14162869.
2
Production and properties of adhesin-free gingipain proteinase RgpA.无黏附素牙龈蛋白酶 RgpA 的生产和性质。
Sci Rep. 2023 Jul 4;13(1):10780. doi: 10.1038/s41598-023-37534-x.
3
Latency, thermal stability, and identification of an inhibitory compound of mirolysin, a secretory protease of the human periodontopathogen .潜伏期、热稳定性及人牙周病原菌分泌性蛋白酶糜蛋白酶抑制剂的鉴定。
J Enzyme Inhib Med Chem. 2021 Dec;36(1):1267-1281. doi: 10.1080/14756366.2021.1937619.
4
Meprin β induces activities of A disintegrin and metalloproteinases 9, 10, and 17 by specific prodomain cleavage.Meprin β 通过特异性前导序列切割诱导解整合素金属蛋白酶 9、10 和 17 的活性。
FASEB J. 2019 Nov;33(11):11925-11940. doi: 10.1096/fj.201801371R. Epub 2019 Aug 9.
5
Proteolytic processing and activation of gingipain zymogens secreted by T9SS of Porphyromonas gingivalis.牙龈卟啉单胞菌 T9SS 分泌的牙龈蛋白酶原的蛋白水解加工和激活。
Biochimie. 2019 Nov;166:161-172. doi: 10.1016/j.biochi.2019.06.010. Epub 2019 Jun 15.
6
Structural determinants of inhibition of Porphyromonas gingivalis gingipain K by KYT-36, a potent, selective, and bioavailable peptidase inhibitor.牙龈卟啉单胞菌牙龈蛋白酶 K 的结构抑制决定因素 KYT-36，一种有效、选择性和生物可用的肽酶抑制剂。
Sci Rep. 2019 Mar 20;9(1):4935. doi: 10.1038/s41598-019-41354-3.
7
The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.IX型分泌系统（T9SS）：其结构与功能的要点及最新见解
Front Cell Infect Microbiol. 2017 May 26;7:215. doi: 10.3389/fcimb.2017.00215. eCollection 2017.
8
Purification and characterisation of recombinant His-tagged RgpB gingipain from Porphymonas gingivalis.牙龈卟啉单胞菌重组组氨酸标签RgpB牙龈蛋白酶的纯化与鉴定
Biol Chem. 2015 Apr;396(4):377-84. doi: 10.1515/hsz-2014-0304.
9
Structure and mechanism of cysteine peptidase gingipain K (Kgp), a major virulence factor of Porphyromonas gingivalis in periodontitis.半胱氨酸蛋白酶牙龈蛋白酶K（Kgp）的结构与机制，牙龈卟啉单胞菌在牙周炎中的一种主要毒力因子
J Biol Chem. 2014 Nov 14;289(46):32291-32302. doi: 10.1074/jbc.M114.602052. Epub 2014 Sep 29.
10
Strategies for the inhibition of gingipains for the potential treatment of periodontitis and associated systemic diseases.抑制gingipains 的策略用于牙周炎和相关系统性疾病的潜在治疗。
J Oral Microbiol. 2014 Aug 18;6. doi: 10.3402/jom.v6.24800. eCollection 2014.
本文引用的文献
1
The bcp gene in the bcp-recA-vimA-vimE-vimF operon is important in oxidative stress resistance in Porphyromonas gingivalis W83.bcp-recA-vimA-vimE-vimF 操纵子中的 bcp 基因在牙龈卟啉单胞菌 W83 的氧化应激抗性中起重要作用。
Mol Oral Microbiol. 2011 Feb;26(1):62-77. doi: 10.1111/j.2041-1014.2010.00596.x. Epub 2010 Nov 18.
2
Dichotomy of gingipains action as virulence factors: from cleaving substrates with the precision of a surgeon's knife to a meat chopper-like brutal degradation of proteins.牙龈蛋白酶作为毒力因子作用的二分法：从像外科手术刀一样精准切割底物到像剁肉刀一样对蛋白质进行粗暴降解。
Periodontol 2000. 2010 Oct;54(1):15-44. doi: 10.1111/j.1600-0757.2010.00377.x.
3
Is the prevalence of periodontitis in the USA in decline?美国牙周炎的患病率在下降吗？
Periodontol 2000. 2009;50:13-24. doi: 10.1111/j.1600-0757.2008.00284.x.
4
The RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis Inactivate the Th2 cytokines interleukin-4 and interleukin-5.牙龈卟啉单胞菌的RgpA-Kgp蛋白酶-黏附素复合物可使Th2细胞因子白细胞介素-4和白细胞介素-5失活。
Infect Immun. 2009 Apr;77(4):1451-8. doi: 10.1128/IAI.01377-08. Epub 2009 Jan 21.
5
Porphyromonas gingivalis RgpA-Kgp proteinase-adhesin complexes penetrate gingival tissue and induce proinflammatory cytokines or apoptosis in a concentration-dependent manner.牙龈卟啉单胞菌RgpA-Kgp蛋白酶-黏附素复合物可穿透牙龈组织，并以浓度依赖的方式诱导促炎细胞因子或细胞凋亡。
Infect Immun. 2009 Mar;77(3):1246-61. doi: 10.1128/IAI.01038-08. Epub 2008 Dec 29.
6
The role of the RgpA-Kgp proteinase-adhesin complexes in the adherence of Porphyromonas gingivalis to fibroblasts.牙龈卟啉单胞菌RgpA-Kgp蛋白酶-黏附素复合物在其黏附成纤维细胞中的作用
Microbiology (Reading). 2008 Oct;154(Pt 10):2904-2911. doi: 10.1099/mic.0.2008/019943-0.
7
Characterization of T cell responses to the RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis in BALB/c mice.BALB/c小鼠中T细胞对牙龈卟啉单胞菌RgpA-Kgp蛋白酶-黏附素复合物反应的特征分析
J Immunol. 2008 Sep 15;181(6):4150-8. doi: 10.4049/jimmunol.181.6.4150.
8
Does the importance of the C-terminal residues in the maturation of RgpB from Porphyromonas gingivalis reveal a novel mechanism for protein export in a subgroup of Gram-Negative bacteria?牙龈卟啉单胞菌RgpB成熟过程中C末端残基的重要性是否揭示了革兰氏阴性菌一个亚群中蛋白质输出的新机制？
J Bacteriol. 2007 Feb;189(3):833-43. doi: 10.1128/JB.01530-06. Epub 2006 Dec 1.
9
Characterization of proteinase-adhesin complexes of Porphyromonas gingivalis.牙龈卟啉单胞菌蛋白酶-黏附素复合物的特性
Microbiology (Reading). 2006 Aug;152(Pt 8):2381-2394. doi: 10.1099/mic.0.28787-0.
10
Activity-based probes that target diverse cysteine protease families.靶向多种半胱氨酸蛋白酶家族的基于活性的探针。
Nat Chem Biol. 2005 Jun;1(1):33-8. doi: 10.1038/nchembio707. Epub 2005 May 24.
Zotero 插件