Jeya Marimuthu, Kim Tae-Su, Tiwari Manish Kumar, Li Jinglin, Zhao Huimin, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, Seoul 143-701, Korea.
Mol Biosyst. 2012 Nov;8(11):2864-7. doi: 10.1039/c2mb25282a. Epub 2012 Sep 4.
BPKS from Botrytis cinerea is a novel type III polyketide synthase that accepts C(4)-C(18) aliphatic acyl-CoAs and benzoyl-CoA as the starters to form pyrones, resorcylic acids and resorcinols through sequential condensation with malonyl-CoA. The catalytic efficiency (k(cat)/K(m)) of BPKS was 2.8 × 10(5) s(-1) M(-1) for palmitoyl-CoA, the highest ever reported. Substrate docking analyses addressed the unique features of BPKS such as its high activity and high specificity toward long chain acyl-CoAs.
来自灰葡萄孢的BPKS是一种新型III型聚酮合酶，它以C(4)-C(18)脂肪族酰基辅酶A和苯甲酰辅酶A作为起始物，通过与丙二酰辅酶A依次缩合形成吡喃酮、间苯二酚酸和间苯二酚。BPKS对棕榈酰辅酶A的催化效率（k(cat)/K(m)）为2.8×10(5) s(-1) M(-1)，这是迄今为止报道的最高值。底物对接分析揭示了BPKS的独特特征，例如它对长链酰基辅酶A具有高活性和高特异性。
