Hussain Ahmed, Bessho Kazuhisa, Takahashi Katsu, Tabata Yasuhiko
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, Japan.
J Tissue Eng Regen Med. 2014 Nov;8(11):919-24. doi: 10.1002/term.1596. Epub 2012 Sep 4.
Gelatin scaffolds incorporating or not 50 wt% of magnesium calcium phosphate (MCP) or β-tricalcium phosphate (βTCP) were prepared and the in vitro osteogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs) in the scaffolds was investigated. The pore sizes of the scaffolds were in the range 123.8 ± 47.2-153 ± 60.72 µm in diameter, while the porosity was 33.3 ± 2-44.9 ± 3.4%. The compression modulus of the sponges was about 2.04-2.24 mPa. There was no significant difference among groups regarding the physical and mechanical properties. When seeded into the sponges by an agitation method, the MSCs were distributed throughout the scaffold. Higher MSC proliferation was observed for scaffolds incorporating minerals. Following the incubation of MSCs in scaffolds incorporating MCP, the alkaline phosphatase activity was significantly higher at weeks 2, 3 and 4 in comparison with other scaffolds; however, the osteocalcin levels of MSCs did not show significant differences among groups. These findings indicate that MSCs seeded in scaffolds incorporating MCP showed significantly superior biological results in terms of proliferation and osteogenic differentiation in comparison with other scaffold types.
制备了含或不含50 wt%磷酸镁钙（MCP）或β - 磷酸三钙（βTCP）的明胶支架，并研究了大鼠骨髓间充质干细胞（MSCs）在支架中的体外成骨分化。支架的孔径范围为直径123.8±47.2 - 153±60.72 µm，孔隙率为33.3±2 - 44.9±3.4%。海绵的压缩模量约为2.04 - 2.24 mPa。各组在物理和力学性能方面无显著差异。当通过搅拌法将MSCs接种到海绵中时，MSCs分布在整个支架中。观察到含矿物质的支架中MSCs增殖更高。在含MCP的支架中培养MSCs后，与其他支架相比，在第2、3和4周时碱性磷酸酶活性显著更高；然而，MSCs的骨钙素水平在各组之间未显示出显著差异。这些发现表明，与其他类型的支架相比，接种在含MCP支架中的MSCs在增殖和成骨分化方面表现出显著优越的生物学结果。
