School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Anal Chem. 2012 Oct 2;84(19):8364-70. doi: 10.1021/ac301918t. Epub 2012 Sep 19.
Real-time tracking of the dynamic process of virus invasion is crucial to understanding the infection mechanism. For successful tracking, efficient labeling methods are indispensable. In this paper, we report a mild and reliable method for labeling viruses, especially with regard to easily disabled enveloped viruses. The copper-free click chemistry has been used to label enveloped viruses with quantum dots (QDs) by linking virions modified with azide to the QDs derived with dibenzocyclooctynes (DBCO). Both vaccinia virus (VACV) and avian influenza A virus (H9N2) can be specifically and rapidly labeled under mild conditions, with a labeling efficiency of more than 80%. The labeled virions were of intact infectivity, and their fluorescence was strong enough to realize single-virion tracking. Compared to previously reported methods, our method is less destructive, reliable, and universal, without specific requirements for the type and structure of viruses to be labeled, which has laid the foundation for long-term dynamic visualization of virus infection process.
实时跟踪病毒入侵的动态过程对于了解感染机制至关重要。为了成功进行跟踪，高效的标记方法是必不可少的。在本文中，我们报告了一种温和可靠的病毒标记方法，特别是对于容易失活的包膜病毒。我们使用无铜点击化学将带有叠氮化物的病毒颗粒与衍生自二苯并环辛炔（DBCO）的量子点（QDs）连接，从而对包膜病毒进行标记。在温和的条件下，痘苗病毒（VACV）和禽流感病毒（H9N2）都可以被特异性和快速地标记，标记效率超过 80%。标记的病毒颗粒具有完整的感染性，其荧光强度足以实现单病毒跟踪。与之前报道的方法相比，我们的方法破坏性更小、更可靠、更通用，对要标记的病毒的类型和结构没有特殊要求，为长期动态可视化病毒感染过程奠定了基础。
