Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
Drug Chem Toxicol. 2013 Jul;36(3):270-6. doi: 10.3109/01480545.2012.710629. Epub 2012 Sep 5.
Sulfur mustard (SM) is a chemical warfare agent with cytotoxic effect and a tight link to oxidative stress (OS). Depletion of antioxidants is considered as a cause of detrimental consequence and belongs to the important steps leading to cell death. The oxidative injury appearing after SM exposure is not well understood. Nevertheless, identification of the pathological processes would be a good opportunity to establish an efficient therapy. Here, we focused our effort on an estimation of reactive oxygen species homeostasis and apoptotic processes in Wistar rats exposed to 0-160 mg/kg of SM. We assayed antioxidant activity, thiobarbituric acid reactive substances, reduced glutathione/oxidized glutathione, metallothionein, glutathione reductase, glutathione peroxidase, glutathione S-transferase, caspase 3, and glucose in the livers, kidneys, and muscles of the animals. Significant OS, depletion of low-molecular-mass antioxidants, increase in caspase activity, and some other processes related to SM action were determined. Moreover, we infer a principal role of OS in the tested organs.
硫芥（SM）是一种具有细胞毒性作用并与氧化应激（OS）密切相关的化学战剂。抗氧化剂的耗竭被认为是有害后果的原因之一，属于导致细胞死亡的重要步骤。SM 暴露后出现的氧化损伤尚不清楚。然而，确定病理过程将是建立有效治疗方法的好机会。在这里，我们集中精力评估暴露于 0-160mg/kg SM 的 Wistar 大鼠中活性氧稳态和凋亡过程。我们测定了抗氧化活性、硫代巴比妥酸反应物质、还原型谷胱甘肽/氧化型谷胱甘肽、金属硫蛋白、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶、半胱天冬酶 3 和动物肝脏、肾脏和肌肉中的葡萄糖。确定了显著的 OS、低分子量抗氧化剂耗竭、半胱天冬酶活性增加以及与 SM 作用相关的其他一些过程。此外，我们推断 OS 在测试器官中起主要作用。
