Tang Qiongying, Huang Yan, Wang Jinjin, Huang Jing, Wang Zhijian, Peng Zuogang
The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan 430072 , P R China.
Mitochondrial DNA. 2013 Feb;24(1):25-7. doi: 10.3109/19401736.2012.716050. Epub 2012 Sep 5.
The genus Triplophysa (plateau loach) is a highly diverse group in the family Balitoridae with 133 valid species, and they play an important role in zoogeographic study of the Qinghai-Tibet Plateau. In this study, we sequenced the complete mitochondrial genome of a Triplophysa species, Triplophysa bleekeri. Combined with the complete mitogenome sequences of other 10 balitorids, the phylogenetic position of T. bleekeri was estimated. The mitochondrial genome of T. bleekeri is similar to those of the typical vertebrates, 16,568 bp in length, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 non-coding control region (D-loop). In the D-loop region, two pair of motifs, "TACAT" and its reverted and complemented sequence "ATGTA", can be found in the domain of termination-associated sequence; six conserved sequences (CSB-F, CSB-E, CSB-D, and CSB I-III) can be identified by searching some conserved motifs. Phylogenetic analyses showed that the three Triplophysa species clustered together, with T. bleekeri sister to Triplophysa stoliczkai. The two genera Triplophysa and Barbatula formed a sister-group relationship, the genus Lefua located in the intermediate position, and the genus Schistura was in the basal position in the subfamily Nemacheilinae. Further investigations with more Triplophysa species included need to be performed to better understand the evolutionary history of this intriguing genus.
高原鳅属（高原鳅）是条鳅科中一个高度多样化的类群，有133个有效物种，它们在青藏高原的动物地理学研究中发挥着重要作用。在本研究中，我们对一种高原鳅——贝氏高原鳅的线粒体基因组进行了测序。结合其他10种条鳅科鱼类的完整线粒体基因组序列，对贝氏高原鳅的系统发育位置进行了估计。贝氏高原鳅的线粒体基因组与典型脊椎动物的相似，长度为16568 bp，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和1个非编码控制区（D环）。在D环区域，在终止相关序列结构域中可发现两对基序，即“TACAT”及其反向互补序列“ATGTA”；通过搜索一些保守基序可鉴定出6个保守序列（CSB-F、CSB-E、CSB-D和CSB I-III）。系统发育分析表明，三种高原鳅物种聚在一起，贝氏高原鳅是斯氏高原鳅的姐妹种。高原鳅属和突吻沙鳅属形成姐妹群关系，犁头鳅属位于中间位置，腹吸鳅属在条鳅亚科中处于基部位置。需要纳入更多高原鳅物种进行进一步研究，以更好地了解这个有趣类群的进化历史。
