Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Phys Med Biol. 2012 Sep 21;57(18):5777-86. doi: 10.1088/0031-9155/57/18/5777. Epub 2012 Sep 5.
The bones of many terrestrial vertebrates, including humans, are continually altered through an internal process of turnover known as remodeling. This process plays a central role in bone adaptation and disease. The uptake of fluorescent tetracyclines within bone mineral is widely exploited as a means of tracking new tissue formation. While investigation of bone microarchitecture has undergone a dimensional shift from 2D to 3D in recent years, we lack a 3D equivalent to fluorescent labeling. In the current study we demonstrate the ability of synchrotron radiation dual energy K-edge subtraction (KES) imaging to map the 3D distribution of elemental strontium within rat vertebral samples. This approach has great potential for ex vivo analysis of preclinical models and human tissue samples. KES also represents a powerful tool for investigating the pharmokinetics of strontium-based drugs recently approved in many countries around the globe for the treatment of osteoporosis.
包括人类在内的许多陆地脊椎动物的骨骼通过一种称为重塑的内部更新过程不断改变。这个过程在骨骼适应和疾病中起着核心作用。荧光四环素在骨矿物质中的摄取被广泛用于追踪新组织的形成。虽然近年来骨微观结构的研究已经从二维转变为三维，但我们缺乏荧光标记的三维等效物。在本研究中，我们展示了同步辐射双能 K 边差减（KES）成像技术在大鼠椎骨样本中元素锶的三维分布的映射能力。这种方法非常适合临床前模型和人体组织样本的离体分析。KES 还代表了一种强大的工具，可用于研究最近在全球许多国家批准用于治疗骨质疏松症的锶基药物的药代动力学。
