Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, PR China.
Oncol Rep. 2012 Nov;28(5):1653-8. doi: 10.3892/or.2012.2011. Epub 2012 Sep 3.
Hepatitis B virus (HBV) infects many individuals globally each year. Researchers usually focus on the relationship between HBV and liver diseases. In this study, we investigated the effects of HBV infection on gastric mucosa. We detected the levels of HBX protein and mRNA in specimens from sixty-four chronic hepatitis B patients (CHB) with gastric ulcers. We confirmed that HBX could aggravate gastric ulcers according to clinicopathological parameters. In addition, we constructed the pcDNA3.1-HBX plasmid and transfected it into GES-1, a gastric mucosal cell line. The results indicated that HBX could induce apoptosis and G1 arrest in GES-1 cells. Insights into the mechanism of HBX action in GES-1 cells were obtained using western blot analysis.
乙型肝炎病毒 (HBV) 每年在全球感染许多个体。研究人员通常专注于 HBV 与肝脏疾病之间的关系。在这项研究中，我们研究了 HBV 感染对胃黏膜的影响。我们检测了 64 例慢性乙型肝炎患者（CHB）合并胃溃疡标本中的 HBX 蛋白和 mRNA 水平。我们根据临床病理参数证实 HBX 可加重胃溃疡。此外，我们构建了 pcDNA3.1-HBX 质粒并转染到胃黏膜细胞系 GES-1 中。结果表明，HBX 可诱导 GES-1 细胞凋亡和 G1 期阻滞。通过 Western blot 分析获得了 HBX 在 GES-1 细胞中作用机制的见解。
