万古霉素耐药粪肠球菌高水平达托霉素耐药性进化的基因表型评估。
Genotypic and phenotypic evaluation of the evolution of high-level daptomycin nonsusceptibility in vancomycin-resistant Enterococcus faecium.
机构信息
Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
出版信息
Antimicrob Agents Chemother. 2012 Nov;56(11):6051-3. doi: 10.1128/AAC.01318-12. Epub 2012 Sep 4.
Abstract
Whole-genome sequencing and cell membrane studies of three clonal Enterococcus faecium strains with daptomycin MICs of 4, 32, and 192 μg/ml were performed, revealing nonsynonymous single nucleotide variants in eight open reading frames, including those predicted to encode a phosphoenolpyruvate-dependent, mannose-specific phosphotransferase system, cardiolipin synthetase, and EzrA. Membrane studies revealed a higher net surface charge among the daptomycin-nonsusceptible isolates and increased septum formation in the isolate with a daptomycin MIC of 192 μg/ml.
摘要
对 3 株万古霉素 MIC 值分别为 4、32 和 192μg/ml 的粪肠球菌进行全基因组测序和细胞膜研究，发现 8 个开放阅读框中有非同义单核苷酸变异，包括预测编码磷酸烯醇丙酮酸依赖性、甘露糖特异性磷酸转移酶系统、心磷脂合成酶和 EzrA 的基因。细胞膜研究显示，万古霉素耐药分离株的净表面电荷更高，且万古霉素 MIC 值为 192μg/ml 的分离株中隔膜形成增加。
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