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Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate.伯克霍尔德氏菌 UDP-葡萄糖脱氢酶（UGD）BceC 的结构及 Tyr10 在 UGD 硫酯中间产物的最终水解中的作用。
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Conformational change upon product binding to Klebsiella pneumoniae UDP-glucose dehydrogenase: a possible inhibition mechanism for the key enzyme in polymyxin resistance.产物与肺炎克雷伯氏菌 UDP-葡萄糖脱氢酶结合导致的构象变化：多粘菌素耐药关键酶的一种可能抑制机制。
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REFMAC5 for the refinement of macromolecular crystal structures.用于大分子晶体结构精修的REFMAC5
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Features and development of Coot.Coot的特点与发展
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嗜热古菌冰岛嗜火菌UDP-葡萄糖脱氢酶的结构

Structure of a UDP-glucose dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum.

作者信息

Sakuraba Haruhiko, Kawai Tomoyuki, Yoneda Kazunari, Ohshima Toshihisa

机构信息

Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Kita-gun, Kagawa 761-0795, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep 1;68(Pt 9):1003-7. doi: 10.1107/S1744309112030667. Epub 2012 Aug 29.

DOI:10.1107/S1744309112030667
PMID:22949183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3433186/
Abstract

The crystal structure of an extremely thermostable UDP-glucose dehydrogenase (UDP-GDH) from the hyperthermophilic archaeon Pyrobaculum islandicum was determined at a resolution of 2.0 Å. The overall fold was comprised of an N-terminal NAD(+) dinucleotide binding domain and a C-terminal UDP-sugar binding domain connected by a long α-helix, and the main-chain coordinates of the enzyme were similar to those of previously studied UDP-GDHs, including the enzymes from Burkholderia cepacia, Streptococcus pyogenes and Klebsiella pneumoniae. However, the sizes of several surface loops in P. islandicum UDP-GDH were much smaller than the corresponding loops in B. cepacia UDP-GDH but were comparable to those of the S. pyogenes and K. pneumoniae enzymes. Structural comparison revealed that the presence of extensive intersubunit hydrophobic interactions, as well as the formation of an intersubunit aromatic pair network, is likely to be the main factor contributing to the hyperthermostability of P. islandicum UDP-GDH.

摘要

对嗜热古菌冰岛嗜火菌中一种极其耐热的尿苷二磷酸葡萄糖脱氢酶（UDP-GDH）的晶体结构进行了测定，分辨率为2.0 Å。其整体折叠结构由一个N端NAD(+)二核苷酸结合结构域和一个C端UDP-糖结合结构域组成，二者由一条长α螺旋连接，该酶的主链坐标与先前研究的UDP-GDH相似，包括洋葱伯克霍尔德菌、化脓性链球菌和肺炎克雷伯菌中的酶。然而，冰岛嗜火菌UDP-GDH中几个表面环的大小比洋葱伯克霍尔德菌UDP-GDH中相应的环小得多，但与化脓性链球菌和肺炎克雷伯菌的酶相当。结构比较表明，广泛的亚基间疏水相互作用以及亚基间芳香族对网络的形成可能是导致冰岛嗜火菌UDP-GDH具有高热稳定性的主要因素。