葡萄（Vitis vinifera）岩藻糖激酶：GDP-岩藻糖焦磷酸化酶的结晶及X射线初步表征
Crystallization and preliminary X-ray characterization of the Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase.
作者信息
Quirk Stephen
机构信息
Kimberly Clark Corp., 1400 Holcomb Bridge Road, Roswell, GA 30022, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep 1;68(Pt 9):1109-12. doi: 10.1107/S1744309112033155. Epub 2012 Aug 31.
Abstract
The Vitis vinifera dual-activity fucose and nucleotide-sugar metabolizing enzyme L-fucokinase:GDP-fucose pyrophosphorylase (FKP) has been purified to homogeneity and the 118.8 kDa monomeric protein has been crystallized by vapor diffusion in Zeppezauer tubes at 277 K. Crystals of the apoenzyme diffracted to 2.6 Å resolution and belonged to the tetragonal space group P4(1)2(1)2. There is a single FKP monomer in the asymmetric unit, giving a Matthews coefficient of 3.22 Å(3) Da(-1) and a solvent content of 61.8%. A complete native data set has been collected as a first step in determining the three-dimensional structure of this enzyme.
摘要
葡萄（Vitis vinifera）双活性岩藻糖和核苷酸糖代谢酶L-岩藻糖激酶：GDP-岩藻糖焦磷酸化酶（FKP）已被纯化至同质，并且该118.8 kDa的单体蛋白已通过在277 K的Zeppezauer管中气相扩散法结晶。脱辅基酶的晶体衍射分辨率达到2.6 Å，属于四方晶系空间群P4(1)2(1)2。不对称单元中有一个FKP单体，马修斯系数为3.22 Å(3) Da(-1)，溶剂含量为61.8%。作为确定该酶三维结构的第一步，已收集了完整的天然数据集。
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