Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Electrophoresis. 2012 Nov;33(21):3229-35. doi: 10.1002/elps.201200223. Epub 2012 Sep 5.
A novel optical microflow cytometer based on external total reflection comprising a laser-induced fluorescence system, a PDMS chip, a plane mirror and a dichroic beamsplitter is proposed for the simultaneous detection, enumeration and sizing of labeled and nonlabeled microparticles. In the proposed approach, the total number and size of the particles passing through the detection region is determined via the nonscattered light signal reflected from a plane mirror positioned over the microchip, while the number of fluorescence-labeled particles is determined via the back scattered fluorescence signal. The experimental results confirm the ability of the proposed system to count and size fluorescent and nonfluorescent particles with nominal diameters ranging from 6 to 10.2 μm. In addition, it is shown that for a mixed sample containing both labeled and nonlabeled particles, the number of nonlabeled particles can be determined by subtracting the number of peaks in the fluorescence signal from that in the reflected light signal.
一种新型的基于外部全反射的光学微流控芯片，包括一个激光诱导荧光系统、一个 PDMS 芯片、一个平面反射镜和一个二向色分光镜，用于同时检测、计数和测量标记和非标记的微粒子。在提出的方法中，通过位于微芯片上方的平面反射镜反射的非散射光信号确定通过检测区域的粒子的总数和大小，而通过背散射荧光信号确定荧光标记粒子的数量。实验结果证实了该系统能够计数和测量标称直径为 6 至 10.2μm 的荧光和非荧光粒子的能力。此外，还表明对于包含标记和非标记粒子的混合样品，可以通过从反射光信号中的峰值数中减去荧光信号中的峰值数来确定非标记粒子的数量。
