Kim Keesung, Jung Ho-Sup, Song Jae-Young, Lee Man-Ryul, Kim Kye-Seong, Suh Kahp-Yang
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.
Electrophoresis. 2009 Sep;30(18):3206-11. doi: 10.1002/elps.200900136.
We present real-time, rapid detection of Mycoplasma pneumonia in PBS inside a Y-channel PDMS microfluidic device via optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed with serially diluted M. pneumonia solutions using highly carboxylated polystyrene particles of 390 and 500 nm diameter conjugated with monoclonal anti-M. pneumonia. Proximity optical fibers were located around the viewing cell of the device, which were used to measure the increase in 45 degrees forward light scattering of the aggregated particles. The detection limit are slightly less than 50 pg mL(-1) both for 390 and 500 nm microspheres and the detection time do not exceed 90 s.
我们通过对乳胶免疫凝集反应进行光纤监测，在Y型通道聚二甲基硅氧烷（PDMS）微流控装置内的磷酸盐缓冲盐水（PBS）中实现了肺炎支原体的实时快速检测。使用直径为390和500 nm的高度羧化聚苯乙烯颗粒与抗肺炎支原体单克隆抗体偶联，对系列稀释的肺炎支原体溶液进行乳胶免疫凝集试验。在该装置的观察池周围布置了近场光纤，用于测量聚集颗粒45度前向光散射的增加。对于390和500 nm的微球，检测限略低于50 pg mL(-1)，检测时间不超过90秒。
