Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83 Huddinge, Sweden.
J Comp Neurol. 2013 Apr 1;521(5):1093-118. doi: 10.1002/cne.23221.
During development of the early neural tube, positional information provided by signaling gradients is translated into a grid of transverse and longitudinal transcription factor expression domains. Transcription factor specification codes defining distinct histogenetic domains within this grid are evolutionarily conserved across vertebrates and may reflect an underlying common vertebrate bauplan. When compared to the rich body of comparative gene expression studies of tetrapods, there is considerably less comparative data available for teleost fish. We used sensitive multicolor fluorescent in situ hybridization to generate a detailed map of regulatory gene expression domains in the embryonic zebrafish diencephalon. The high resolution of this technique allowed us to resolve abutting and overlapping gene expression of different transcripts. We found that the relative topography of gene expression patterns in zebrafish was highly similar to those of orthologous genes in tetrapods and consistent with a three-prosomere organization of the alar and basal diencephalon. Our analysis further demonstrated a conservation of intraprosomeric subdivisions within prosomeres 1, 2, and 3 (p1, p2, and p3). A tripartition of zebrafish p1 was identified reminiscent of precommissural (PcP), juxtacommissural (JcP), and commissural (CoP) pretectal domains of tetrapods. The constructed detailed diencephalic transcription factor gene expression map further identified molecularly distinct thalamic and prethalamic rostral and caudal domains and a prethalamic eminence histogenetic domain in zebrafish. Our comparative gene expression analysis conformed with the idea of a common bauplan for the diencephalon of anamniote and amniote vertebrates from fish to mammals.
在早期神经管发育过程中，信号梯度提供的位置信息被转化为横向和纵向转录因子表达域的网格。在这个网格中，定义不同组织发生域的转录因子规范代码在脊椎动物中是保守的，可能反映了一个潜在的共同脊椎动物体式计划。与四足动物丰富的比较基因表达研究相比，关于硬骨鱼的比较数据要少得多。我们使用灵敏的多色荧光原位杂交技术，生成了胚胎斑马鱼间脑的调控基因表达域的详细图谱。该技术的高分辨率使我们能够解析不同转录本的相邻和重叠的基因表达。我们发现，斑马鱼基因表达模式的相对拓扑结构与四足动物的同源基因非常相似，并且与翼状和基底间脑的三个体节组织一致。我们的分析进一步证明了 1 号、2 号和 3 号前体节内的内前体节细分（p1、p2 和 p3）的保守性。鉴定出斑马鱼 p1 的三分体类似于四足动物的前连合（PcP）、近连合（JcP）和连合（CoP）前视区。构建的详细间脑转录因子基因表达图谱进一步确定了在斑马鱼中分子上不同的丘脑和视前颅侧和尾侧域以及视前隆起组织发生域。我们的比较基因表达分析符合从鱼类到哺乳动物的无颌类和有颌类脊椎动物间脑具有共同体式计划的观点。
