Gastrointestinal and Liver Pathology Research Laboratory, Department of Pathology, Karolinska Institute and University Hospital, 17176, Stockholm, Sweden.
In Vivo. 2012 Sep-Oct;26(5):859-62.
Caeliac disease is a common immune-mediated condition in the proximal small intestine, generated by a permanent intolerance to cereal gluten proteins in genetically predisposed individuals. It has become apparent that abnormal microbiota proliferate in the duodenal lumen of patients with caeliac disease. Recently it was also noticed that an antibody against multiple myeloma oncogene 1/IRF4 (MUM1) stained plasma cells and their precursors.
Eleven consecutive duodenal biopsies were investigated; four had villous atrophy (caeliac patients) and the remaining seven exhibited histologically normal mucosa (non-caeliac patients). Sections were stained with H&E and with anti-MUM1. A graticulated eyepiece (10 mm, divided into 10 × 10 squares) was used for counting of MUM1-expressing cells in the superficial compartment (SC) and in the deep compartment (DC) of the lamina propria mucosa (lpm).
In the duodenal mucosa of caeliac patients the mean number of MUM1-labelled cells in 12 areas of the lpm was 67.1 (range 37-88) in the SC and 61.5 (range 42-84) in the DC. In the duodenal mucosa of non-caeliac patients, the mean number of MUM1-labelled cells in 21 areas of the lpm was 7.6 (range 0-24) in the SC, and 29.2 (range 22-40) in the DC (p<0.05).
These preliminary results showed that a significantly higher number of plasma cells/plasma cell precursors accumulate in the lpm in patients with caeliac disease, particularly in the SC. This abnormal accumulation of MUM1-expressing cells might be a defence mechanism against the alien bacterial flora recently reported in the duodenal microenvironment in caeliac patients. This appears to be the first report in which MUM1 immunostaining is applied to assess the frequency of plasma cell precursors in the duodenal mucosa in caeliac patients.
腹腔疾病是一种常见的近端小肠免疫介导疾病，由遗传易感性个体对谷类麸质蛋白的永久性不耐受引起。目前已经明显发现，异常微生物群在腹腔疾病患者的十二指肠腔内增殖。最近还注意到，针对多发性骨髓瘤致癌基因 1/IRF4（MUM1）的抗体可染色浆细胞及其前体。
研究了 11 例连续的十二指肠活检，其中 4 例有绒毛萎缩（腹腔疾病患者），其余 7 例表现为组织学正常的黏膜（非腹腔疾病患者）。对切片进行 H&E 和抗 MUM1 染色。使用带有 10 毫米网格的目镜（分为 10×10 个正方形），对固有层黏膜浅层（SC）和深层（DC）的 MUM1 表达细胞进行计数。
在腹腔疾病患者的十二指肠黏膜中，固有层黏膜 12 个区域的 MUM1 标记细胞的平均值在 SC 中为 67.1（范围 37-88），在 DC 中为 61.5（范围 42-84）。在非腹腔疾病患者的十二指肠黏膜中，固有层黏膜 21 个区域的 MUM1 标记细胞的平均值在 SC 中为 7.6（范围 0-24），在 DC 中为 29.2（范围 22-40）（p<0.05）。
这些初步结果表明，腹腔疾病患者固有层黏膜中浆细胞/浆细胞前体的数量明显增加，特别是在 SC 中。MUM1 表达细胞的这种异常积聚可能是针对最近在腹腔疾病患者十二指肠微环境中报道的外来细菌菌群的防御机制。这似乎是首次应用 MUM1 免疫染色来评估腹腔疾病患者十二指肠黏膜中浆细胞前体频率的报道。
