Itoh Shousaku, Matsushita Kenta, Ikeda Shun, Yamamoto Yumiko, Yamauchi Yukako, Yoshioka Seisuke, Yamamoto Reiko, Ebisu Shigeyuki, Hayashi Mikako, Aubin Jane E
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Room 4245, Medical Sciences Building, 1 King's College Circle, Toronto, ON M5S 1A8, Canada.
Int J Mol Sci. 2012;13(8):10229-10235. doi: 10.3390/ijms130810229. Epub 2012 Aug 16.
We recently succeeded in purifying a novel multipotential progenitor or stem cell population from bone marrow stromal cells (BMSCs). This population exhibited a very high frequency of colony forming units-osteoblast (CFU-O; 100 times higher than in BMSCs) and high expression levels of osteoblast differentiation markers. Furthermore, large masses of mineralized tissue were observed in in vivo transplants with this new population, designated highly purified osteoprogenitors (HipOPs). We now report the detailed presence and localization of HipOPs and recipient cells in transplants, and demonstrate that there is a strong relationship between the mineralized tissue volume formed and the transplanted number of HipOPs.