Miyakoshi J, Dobler K D, Allalunis-Turner J, McKean J D, Petruk K, Allen P B, Aronyk K N, Weir B, Huyser-Wierenga D, Fulton D
Department of Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.
Cancer Res. 1990 Jan 15;50(2):278-83.
We report that 5 of 19 human malignant glioma cell lines have neither interferon alpha (IFNA) nor interferon beta (IFNB) genes that are detectable by Southern blotting. Of 5 other of these malignant glioma lines that have a single IFNB gene copy, 3 lack the IFNA genes entirely and two have one copy. One of the lines that lacks the IFNA genes entirely but has one copy of the IFNB gene has a rearrangement near the IFNB gene that is most easily interpreted as an insertion of a large segment of DNA (at least 50 kilobases) the 3' end of which is less than 1.3 kilobases 5' to the known regulatory sequences of the IFNB gene. In spite of the rearrangement, IFNB-specific RNA is highly inducible in this line by poly(I)-poly(C). The ability of interferon alpha or interferon beta to inhibit cell growth does not depend upon the presence or absence of the respective gene. This finding adds solid tumors to those tumor cell lines (acute lymphocytic leukemia, chronic myelogeneous leukemia) previously determined to lack the IFNA and IFNB genes (Diaz et al., Proc. Natl. Acad. Sci. USA, 85:5259-5263, 1988).
我们报告，19个人类恶性胶质瘤细胞系中有5个通过Southern印迹法检测不到干扰素α（IFNA）和干扰素β（IFNB）基因。在这些恶性胶质瘤细胞系中，另外5个有单个IFNB基因拷贝，其中3个完全缺乏IFNA基因，2个有一个拷贝。完全缺乏IFNA基因但有一个IFNB基因拷贝的细胞系之一，在IFNB基因附近有一个重排，最容易解释为插入了一大段DNA（至少50千碱基），其3'端距离IFNB基因已知调控序列的5'端小于1.3千碱基。尽管有重排，该细胞系中IFNB特异性RNA仍可被聚肌苷酸-聚胞苷酸高度诱导。干扰素α或干扰素β抑制细胞生长的能力并不取决于各自基因的有无。这一发现使实体瘤也加入到先前确定缺乏IFNA和IFNB基因的肿瘤细胞系（急性淋巴细胞白血病、慢性粒细胞白血病）中（迪亚兹等人，《美国国家科学院院刊》，85:5259 - 5263，1988）。
