Koszykowska Marlena, Całka Jarosław, Nidzgorska Aleksandra, Jana Barbara
Division of Biology Reproduction, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn 10-747, Tuwima 10, Poland.
Reprod Fertil Dev. 2013;25(4):661-73. doi: 10.1071/RD11271.
The aim of the present study was to determine the effect of long-term 17β-oestradiol (E2) exposure, a simulation of pathological states that occur with oestrogen overproduction, on the innervation patterns of ovaries in adult gilts. The intraovarian distribution and density of nerve fibres immunoreactive (IR) to protein gene product (PGP) 9.5 and containing dopamine-β-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM) and galanin (GAL) were determined. From Day 4 of the first oestrous cycle to Day 20 of the second cycle studied, experimental gilts were injected with E2 (1000μg every 12h) whereas control gilts were injected with corn oil. After E2 administration, there was an increase in the number of PGP9.5-, DBH-, NPY- and GAL-IR fibres. Numerous PGP9.5-IR terminals were observed within the ground plexus around secondary follicles and small or medium tertiary follicles. Long-term E2 treatment increased the density of DBH- and NPY-IR fibres in the cortical part of the ground plexus, DBH- and GAL-IR fibres in the medullary part of the ground plexus, DBH-IR fibres near small and medium tertiary follicles and NPY-IR fibres around medullary arteries. The data indicate that long-term exposure of gilts to E2 increases the total number of intraovarian fibres, including sympathetic fibres. These results suggest that elevated E2 levels that occur during pathological states may affect the innervation patterns of ovaries and their function(s).
本研究的目的是确定长期暴露于17β-雌二醇（E2）（模拟雌激素分泌过多时出现的病理状态）对成年后备母猪卵巢神经支配模式的影响。测定了卵巢内对蛋白基因产物（PGP）9.5免疫反应（IR）且含有多巴胺-β-羟化酶（DBH）、神经肽Y（NPY）、生长抑素（SOM）和甘丙肽（GAL）的神经纤维的分布和密度。从第一个发情周期的第4天到所研究的第二个周期的第20天，给实验母猪注射E2（每12小时1000μg），而对照母猪注射玉米油。给予E2后，PGP9.5、DBH、NPY和GAL免疫反应纤维的数量增加。在次级卵泡和中小三级卵泡周围的基层神经丛内观察到大量PGP9.5免疫反应终末。长期E2处理增加了基层神经丛皮质部分DBH和NPY免疫反应纤维的密度、基层神经丛髓质部分DBH和GAL免疫反应纤维的密度、中小三级卵泡附近DBH免疫反应纤维的密度以及髓质动脉周围NPY免疫反应纤维的密度。数据表明，后备母猪长期暴露于E2会增加卵巢内纤维的总数，包括交感神经纤维。这些结果表明，病理状态下升高的E2水平可能会影响卵巢的神经支配模式及其功能。
