Wojtkiewicz Joanna, Jana Barbara, Kozłowska Anna, Crayton Robert, Majewski Mariusz, Zalecki Michał, Baranowski Włodzimierz, Radziszewski Piotr
Department of Pathophysiology, Laboratory for Regenerative Medicine, Stem Cell Research Laboratory, Department of Neurology and Neurosurgery University of Warmia and Mazury, Olsztyn, Poland.
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland.
J Chem Neuroanat. 2014 Nov;61-62:147-52. doi: 10.1016/j.jchemneu.2014.05.003. Epub 2014 Jun 4.
The aim of the present study was to determine the changes in both the distribution pattern and density of nerve fibers containing dopamine β-hydroxylase (DβH), vesicular acetylcholine transporter (VAChT), neuronal nitric oxide synthase (nNOS), substance P (SP), calcitonin gene related peptide (CGRP), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), somatostatin (SOM), galanin (GAL) and pituitary adenylate cyclase-activating polypeptide (PACAP) in the human polycystic ovaries. In the polycystic ovaries, when compared to the immunoreactions pattern observed in the control gonads, following changes were revealed: (1) an increase in the number of DβH-, VAChT-, VIP- or GAL-immunoreactive (IR) nerve fibers within the stroma as well as in the number of DβH-IR fibers near primordial follicles and medullar veins and venules; (2) a reduction in the number of nerve fibers containing nNOS, CGRP, SOM, PACAP within the stroma and in the numbers of CGRP-IR fibers around arteries; (3) an appearance of SP- and GAL-IR fibers around medullar and cortical arteries, arterioles, veins and venules, with except of GAL-IR fibers supplying medullar veins; and (4) the lack of nNOS-IR nerve fibers near primordial follicles and VIP-IR nerves around medullar arteries and arterioles. In conclusion, our results suggest that the changes in the innervation pattern of the polycystic ovaries in human may play an important role in the pathogenesis and/or course of this disorder.
本研究的目的是确定人多囊卵巢中含多巴胺β-羟化酶（DβH）、囊泡乙酰胆碱转运体（VAChT）、神经元型一氧化氮合酶（nNOS）、P物质（SP）、降钙素基因相关肽（CGRP）、神经肽Y（NPY）、血管活性肠肽（VIP）、生长抑素（SOM）、甘丙肽（GAL）和垂体腺苷酸环化酶激活多肽（PACAP）的神经纤维分布模式和密度的变化。在多囊卵巢中，与对照性腺中观察到的免疫反应模式相比，发现了以下变化：（1）基质内以及原始卵泡和髓质静脉及小静脉附近DβH-、VAChT-、VIP-或GAL-免疫反应性（IR）神经纤维数量增加；（2）基质内含有nNOS、CGRP、SOM、PACAP的神经纤维数量以及动脉周围CGRP-IR纤维数量减少；（3）在髓质和皮质动脉、小动脉、静脉和小静脉周围出现SP-和GAL-IR纤维，但供应髓质静脉的GAL-IR纤维除外；（4）原始卵泡附近缺乏nNOS-IR神经纤维，髓质动脉和小动脉周围缺乏VIP-IR神经。总之，我们的结果表明，人类多囊卵巢神经支配模式的变化可能在该疾病的发病机制和/或病程中起重要作用。
