成年猪长期接受睾酮治疗会改变卵巢神经支配。

Long-term treatment with testosterone alters ovary innervation in adult pigs.

作者信息

Jana Barbara, Meller Karolina A, Bulc Michał, Całka Jarosław

机构信息

Division of Biology Reproduction, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, 10-748, Poland.

Division of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, 10-718, Poland.

出版信息

J Ovarian Res. 2016 Oct 10;9(1):64. doi: 10.1186/s13048-016-0273-4.

DOI:10.1186/s13048-016-0273-4

PMID:27724935

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5057494/

Abstract

BACKGROUND

Intraovarian distribution and density of nerve fibres immunoreactive (IR) to protein gene product 9.5 (PGP 9.5) and containing dopamine-β-hydroxylase (DβH), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL) were determined.

METHODS

From day 4 of the first oestrous cycle to day 20 of the second studied cycle, experimental gilts (n = 3) were injected with testosterone (T), while control gilts (n = 3) received corn oil.

RESULTS

After T administration the numbers of fibres IR to PGP 9.5 and fibres IR to DβH, NPY and SOM were decreased. Fewer PGP 9.5- and DβH-IR terminals were observed within the ground plexus and around arteries and medullar veins, and medium tertiary follicles, and DβH-IR terminals in the vicinity of small tertiary follicles. T decreased the density of NPY-IR fibres in the medullar part of the ground plexus, and SOM-IR in the cortical part of the ground plexus.

CONCLUSIONS

The obtained data show that long-term T treatment of gilts decreases the total number of intraovarian fibres, including sympathetic ones. These results suggest that elevated T levels that occur during pathological states may affect the innervation pattern of ovaries, and their function(s).

摘要

背景

测定了卵巢内对蛋白基因产物9.5（PGP 9.5）免疫反应（IR）以及含有多巴胺-β-羟化酶（DβH）、神经肽Y（NPY）、生长抑素（SOM）、甘丙肽（GAL）的神经纤维的分布和密度。

方法

从第一个发情周期的第4天到第二个研究周期的第20天，给实验小母猪（n = 3）注射睾酮（T），而对照小母猪（n = 3）注射玉米油。

结果

给予T后，对PGP 9.5免疫反应的纤维数量以及对DβH、NPY和SOM免疫反应的纤维数量减少。在基层神经丛内、动脉和髓质静脉周围以及中等三级卵泡内观察到的PGP 9.5和DβH免疫反应终末较少，在小三级卵泡附近的DβH免疫反应终末也较少。T降低了基层神经丛髓质部分NPY免疫反应纤维的密度以及基层神经丛皮质部分SOM免疫反应纤维的密度。