芳基胺的生物监测：尿素和氨基甲酸酯类农药的血红蛋白加合物

Biomonitoring of arylamines: hemoglobin adducts of urea and carbamate pesticides.

作者信息

Sabbioni G, Neumann H G

机构信息

Institut für Pharmakologie und Toxikologie, Universität Würzburg, FRG.

出版信息

Carcinogenesis. 1990 Jan;11(1):111-5. doi: 10.1093/carcin/11.1.111.

DOI:10.1093/carcin/11.1.111

PMID:2295119

Abstract

Hemoglobin adducts of aromatic amines released from pesticides were investigated. Female Wistar rats were dosed orally with pesticides up to 1 mmol/kg body weight. Blood was obtained after 24 h, hemoglobin isolated and hydrolyzed in 1 NaOH. The amines were extracted and quantified by gas chromatography with nitrogen-specific or mass-selective detection. The following binding indices [HBI, hemoglobin binding index = binding (mmol/mol Hb) per dose (mmol/kg)] were obtained: pesticide (arylamine): linuron, diuron (3,4-dichloroaniline) 0.8 and 4.5 respectively; monuron, monolinuron (4-chloroaniline) 39 and 55 respectively; chlorpropham (3-chloroaniline) 2.9; chlordimeform (4-chloro-o-toluidine) 2.4; propham (aniline) 2.4. With vinclozoline and iprodione (3,5-dichloroaniline) and quintozene (pentachloroaniline) no adducts could be found. The results demonstrate the possible use of arylamine-hemoglobin adducts for measuring the bioavailability of potentially hazardous components of pesticides and the extent to which they are formed and metabolically activated.

摘要

对从农药中释放出的芳香胺的血红蛋白加合物进行了研究。给雌性Wistar大鼠经口给予高达1 mmol/kg体重的农药。24小时后采集血液，分离血红蛋白并在1 N NaOH中水解。通过具有氮特异性或质量选择性检测的气相色谱法提取并定量胺类。获得了以下结合指数[HBI，血红蛋白结合指数=每剂量（mmol/kg）的结合量（mmol/mol Hb）]：农药（芳胺）：利谷隆、敌草隆（3,4-二氯苯胺）分别为0.8和4.5；灭草隆、绿谷隆（4-氯苯胺）分别为39和55；氯苯胺灵（3-氯苯胺）为2.9；杀虫脒（4-氯邻甲苯胺）为2.4；苯胺灵（苯胺）为2.4。对于乙烯菌核利和异菌脲（3,5-二氯苯胺）以及五氯硝基苯（五氯苯胺），未发现加合物。结果表明，芳胺-血红蛋白加合物可用于测量农药潜在有害成分的生物利用度以及它们形成和代谢活化的程度。