Pasco Rachael, Gardner David K, Walker David W, Dickinson Hayley
The Ritchie Centre, Monash Institute of Medical Research, Monash University, Clayton, Vic. 3168, Australia.
Reprod Fertil Dev. 2012;24(8):1117-22. doi: 10.1071/RD12044.
This study aimed to develop a superovulation protocol for the spiny mouse (Acomys cahirinus). The spiny mouse is a desert-adapted rodent species, with a long oestrus cycle (11 days) compared with rat and mouse, and gives birth to few (mean litter size is 3) precocial offspring after a relatively long gestation (39 days). We successfully optimised a superovulation protocol that elicited a 5-fold increase in the normal ovulation rate of this species. To induce superovulation in the spiny mouse 2 injections of equine chorionic gonadotrophin (eCG, 10 IU each), 9h apart, were required, followed by 20 IU of human chorionic gonadotrophin (hCG). This protocol was successful in 100% of females trialed and at 33 h post-hCG an average of 14.7 ± 1.5, 1-2 cell embryos were recovered. Histological analysis of ovaries following superovulation revealed large corpus lutea and post-ovulatory follicles occupying a large part of the ovary. Ovulation commenced 6-12 h after the hCG injection and continued until 24-33 h post-hCG as indicated by both histological analysis of ovaries and the presence of oocytes/embryos in the oviduct. This superovulation protocol will facilitate the development of an in vitro culture system for spiny mouse embryos.
本研究旨在开发一种针对刺毛鼠（Acomys cahirinus）的超排卵方案。刺毛鼠是一种适应沙漠环境的啮齿动物物种，与大鼠和小鼠相比，其发情周期较长（11天），在相对较长的妊娠期（39天）后产下数量较少（平均窝仔数为3只）的早熟后代。我们成功优化了一种超排卵方案，使该物种的正常排卵率提高了5倍。为了诱导刺毛鼠超排卵，需要间隔9小时注射2次马绒毛膜促性腺激素（eCG，每次10 IU），随后注射20 IU人绒毛膜促性腺激素（hCG）。该方案在所有受试雌性中成功率为100%，在注射hCG后33小时，平均回收了14.7±1.5个1-2细胞胚胎。超排卵后卵巢的组织学分析显示，大黄体和排卵后卵泡占据了卵巢的很大一部分。卵巢组织学分析以及输卵管中卵母细胞/胚胎的存在表明，排卵在注射hCG后6-12小时开始，并持续至注射hCG后24-33小时。这种超排卵方案将有助于开发刺毛鼠胚胎的体外培养系统。
