Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
J Virol. 2012 Nov;86(22):12362-71. doi: 10.1128/JVI.01532-12. Epub 2012 Sep 5.
The mRNAs encoding the Rev and Env proteins of simian immunodeficiency virus (SIV) are unique because upstream translation start codons are present that may modulate the expression of these viral proteins. This is true for the regular mRNAs, but we also report novel mRNA splicing variants that encode up to five upstream AUG (uAUG) codons. Their influence on Rev and Env translation was measured by mutational inactivation in reporter constructs and in the SIVmac239 strain. An intricate regulatory mechanism was disclosed that allows the virus to express a balanced amount of these two proteins. This insight also allows the design of vector constructs that efficiently express these proteins.
猿猴免疫缺陷病毒（SIV）的 Rev 和 Env 蛋白的编码 mRNA 是独特的，因为存在可能调节这些病毒蛋白表达的上游翻译起始密码子。这对于常规 mRNA 是正确的，但我们也报告了新的 mRNA 剪接变体，这些变体编码多达五个上游 AUG（uAUG）密码子。它们对 Rev 和 Env 翻译的影响通过在报告构建体和 SIVmac239 株中突变失活来测量。揭示了一种复杂的调节机制，使病毒能够表达这两种蛋白的平衡量。这一见解还允许设计有效地表达这些蛋白的载体构建体。
