Kikhno Irina
Institute of Molecular Biology & Genetics of Ukrainian Academy of Science, Kiev, Ukraine.
PLoS One. 2014 Apr 16;9(4):e95322. doi: 10.1371/journal.pone.0095322. eCollection 2014.
Highly homologous sequences 154-157 bp in length grouped under the name of "conserved non-protein-coding element" (CNE) were revealed in all of the sequenced genomes of baculoviruses belonging to the genus Alphabaculovirus. A CNE alignment led to the detection of a set of highly conserved nucleotide clusters that occupy strictly conserved positions in the CNE sequence. The significant length of the CNE and conservation of both its length and cluster architecture were identified as a combination of characteristics that make this CNE different from known viral non-coding functional sequences. The essential role of the CNE in the Alphabaculovirus life cycle was demonstrated through the use of a CNE-knockout Autographa californica multiple nucleopolyhedrovirus (AcMNPV) bacmid. It was shown that the essential function of the CNE was not mediated by the presumed expression activities of the protein- and non-protein-coding genes that overlap the AcMNPV CNE. On the basis of the presented data, the AcMNPV CNE was categorized as a complex-structured, polyfunctional genomic element involved in an essential DNA transaction that is associated with an undefined function of the baculovirus genome.
在属于甲型杆状病毒属的所有杆状病毒测序基因组中，均发现了长度为154 - 157 bp的高度同源序列，这些序列被归为“保守非蛋白质编码元件”（CNE）。对CNE进行比对后，检测到一组高度保守的核苷酸簇，它们在CNE序列中占据着严格保守的位置。CNE的显著长度以及其长度和簇结构的保守性，被确定为使其与已知病毒非编码功能序列不同的特征组合。通过使用CNE敲除的苜蓿银纹夜蛾多粒包埋核型多角体病毒（AcMNPV）杆粒，证明了CNE在甲型杆状病毒生命周期中的重要作用。结果表明，CNE的基本功能并非由与AcMNPV CNE重叠的蛋白质编码基因和非蛋白质编码基因的假定表达活性介导。根据所提供的数据，AcMNPV CNE被归类为一种复杂结构、多功能的基因组元件，参与了与杆状病毒基因组未定义功能相关的重要DNA交易。
