Sanofi Pasteur, VaxDesign Campus, Orlando, Florida, United States of America.
PLoS One. 2012;7(8):e42681. doi: 10.1371/journal.pone.0042681. Epub 2012 Aug 30.
Considering importance of ganglioside antibodies as biomarkers in various immune-mediated neuropathies and neurological disorders, we developed a high throughput multiplexing tool for the assessment of gangliosides-specific antibodies based on Biolpex/Luminex platform. In this report, we demonstrate that the ganglioside high throughput multiplexing tool is robust, highly specific and demonstrating ∼100-fold higher concentration sensitivity for IgG detection than ELISA. In addition to the ganglioside-coated array, the high throughput multiplexing tool contains beads coated with influenza hemagglutinins derived from H1N1 A/Brisbane/59/07 and H1N1 A/California/07/09 strains. Influenza beads provided an added advantage of simultaneous detection of ganglioside- and influenza-specific antibodies, a capacity important for the assay of both infectious antigen-specific and autoimmune antibodies following vaccination or disease. Taken together, these results support the potential adoption of the ganglioside high throughput multiplexing tool for measuring ganglioside antibodies in various neuropathic and neurological disorders.
鉴于神经节苷脂抗体作为各种免疫介导性神经病和神经紊乱的生物标志物的重要性，我们开发了一种基于 Biolpex/Luminex 平台的高通量多重检测工具，用于评估神经节苷脂特异性抗体。在本报告中，我们证明了这种神经节苷脂高通量多重检测工具具有强大的特异性，并且与 ELISA 相比，对 IgG 的检测灵敏度高 100 倍。除了神经节苷脂包被的微球阵列外，高通量多重检测工具还包含了由 H1N1 A/Brisbane/59/07 和 H1N1 A/California/07/09 株流感血凝素包被的微球。流感微球提供了同时检测神经节苷脂和流感特异性抗体的额外优势，这对于接种疫苗或患病后检测感染性抗原特异性和自身免疫性抗体非常重要。总之，这些结果支持采用神经节苷脂高通量多重检测工具来测量各种神经病变和神经紊乱中的神经节苷脂抗体。
