Key Laboratory in Cell Transplantation in Ministry of Health of China, Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
PLoS One. 2012;7(8):e42813. doi: 10.1371/journal.pone.0042813. Epub 2012 Aug 28.
We aimed to investigate whether an innovative growth factor-laden scaffold composed of acellular sciatic nerve (ASN) and brain-derived neurotrophic factor (BDNF) could promote axonal regeneration and functional recovery after spinal cord injury (SCI).
Following complete transection at the thoracic level (T9), we immediately transplanted the grafts between the stumps of the severed spinal cords. We evaluated the functional recovery of the hindlimbs of the operated rats using the BBB locomotor rating scale system every week. Eight weeks after surgery, axonal regeneration was examined using the fluorogold (FG) retrograde tracing method. Electrophysiological analysis was carried out to evaluate the improvement in the neuronal circuits. Immunohistochemistry was employed to identify local injuries and recovery.
The results of the Basso-Beattie-Bresnahan (BBB) scale indicated that there was no significant difference between the individual groups. The FG retrograde tracing and electrophysiological analyses indicated that the transplantation of ASN-BDNF provided a permissive environment to support neuron regeneration.
The ASN-BDNF transplantation provided a promising therapeutic approach to promote axonal regeneration and recovery after SCI, and can be used as part of a combinatory treatment strategy for SCI management.
我们旨在研究一种由去细胞坐骨神经（ASN）和脑源性神经营养因子（BDNF）组成的创新生长因子支架是否可以促进脊髓损伤（SCI）后轴突再生和功能恢复。
在胸段（T9）完全横断后，我们立即将移植物移植到切断的脊髓残端之间。我们使用 BBB 运动评分系统每周评估手术大鼠后肢的功能恢复情况。手术后 8 周，使用荧光金（FG）逆行示踪法检查轴突再生情况。进行电生理分析以评估神经元回路的改善情况。免疫组织化学用于识别局部损伤和恢复情况。
Basso-Beattie-Bresnahan（BBB）量表的结果表明各组之间没有显著差异。FG 逆行示踪和电生理分析表明，ASN-BDNF 的移植为支持神经元再生提供了许可环境。
ASN-BDNF 移植为促进 SCI 后轴突再生和恢复提供了一种有前途的治疗方法，并可作为 SCI 管理的联合治疗策略的一部分。
