Van Vooren J P, Farber C M, De Bruyn J, Yernault J C
Department of Immunology, Hopital Erasme, Brussels, Belgium.
Chest. 1990 Jan;97(1):88-90. doi: 10.1378/chest.97.1.88.
We devised a dot blot assay to evaluate the IgG and IgA response to P32, a recently isolated antigen specific to mycobacteria. Pleural fluids and the corresponding sera were tested, obtained from five patients with pleural tuberculosis proven by direct examination and/or culture and from 14 patients with pleural effusions of other origins. We measured the total IgG and IgA levels in all samples and determined the anti-P32 titer after adjusting IgG and IgA respectively to the same levels in all samples. Those pleural fluids and sera from tuberculous patients contained a higher proportion of anti-P32 antibodies than samples obtained from nontuberculous control subjects; in those patients, the proportion of anti-P32 antibodies was generally higher in pleural effusion fluid than in serum.
我们设计了一种斑点印迹法，以评估针对P32的IgG和IgA反应，P32是一种最近分离出的分枝杆菌特异性抗原。对5例经直接检查和/或培养证实为胸膜结核的患者以及14例其他病因所致胸腔积液患者的胸腔积液及相应血清进行检测。我们测定了所有样本中的总IgG和IgA水平，并在将所有样本中的IgG和IgA分别调整至相同水平后，测定抗P32滴度。与非结核对照受试者的样本相比，结核患者的胸腔积液和血清中抗P32抗体的比例更高；在这些患者中，胸腔积液中抗P32抗体的比例通常高于血清中的比例。
