Grossman Z, Ram D, Markovics A, Tarrab-Hazdai R, Lantner F, Ziv E, Schechter I
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Exp Parasitol. 1990 Jan;70(1):62-71. doi: 10.1016/0014-4894(90)90086-r.
It was previously shown that an antigen preparation termed 9B obtained from Schistosoma mansoni cercarial extracts partially (34%) protects mice from challenge infection with cercariae (R. Tarrab-Hazdai et al., J. Immunol. 135, 2772, 1985). To characterize some of the proteins which comprise this preparation, rabbit antibodies to the 9B antigen preparation were used to screen cDNA libraries of cercariae and adult worms. We isolated and sequenced cDNA clones encoding three proteins: calcium-binding protein, paramyosin, and myosin. The calcium-binding protein was previously shown to be expressed in cercariae but not in sporocysts or adult worms (D. Ram et al., Mol. Biochem. Parasitol. 34, 167, 1989). Northern blots showed the presence of paramyosin and myosin mRNAs in sporocysts and adult worms but not in cercariae. Antibodies to paramyosin detected the protein in sporocysts and adult worms as well as in cercariae. These findings explain, in part, the protective activity of the 9B antigen preparation against challenge infection.
先前的研究表明，从曼氏血吸虫尾蚴提取物中获得的一种名为9B的抗原制剂可部分（34%）保护小鼠免受尾蚴攻击感染（R. Tarrab-Hazdai等人，《免疫学杂志》135, 2772, 1985）。为了鉴定构成该制剂的一些蛋白质，用针对9B抗原制剂的兔抗体筛选尾蚴和成虫的cDNA文库。我们分离并测序了编码三种蛋白质的cDNA克隆：钙结合蛋白、副肌球蛋白和肌球蛋白。先前已表明钙结合蛋白在尾蚴中表达，但在孢蚴或成虫中不表达（D. Ram等人，《分子生物化学寄生虫学》34, 167, 1989）。Northern印迹显示副肌球蛋白和肌球蛋白mRNA在孢蚴和成虫中存在，但在尾蚴中不存在。针对副肌球蛋白的抗体在孢蚴、成虫以及尾蚴中均检测到了该蛋白质。这些发现部分解释了9B抗原制剂对攻击感染的保护活性。
