Laclette J P, Landa A, Arcos L, Willms K, Davis A E, Shoemaker C B
Department of Immunology, Universidad Nacional Autónoma de México, Mexico City.
Mol Biochem Parasitol. 1991 Feb;44(2):287-95. doi: 10.1016/0166-6851(91)90015-x.
Antigen B, a major antigen of the cestode parasite Taenia solium, has been purified and a portion of amino acid sequence obtained. Paramyosin of the trematode parasite Schistosoma mansoni, an immunogenic protein that has shown promise as a vaccine candidate, has several biochemical and immunological properties in common with antigen B. A full-length cDNA clone of S. mansoni paramyosin has been obtained and the predicted translation product contains a sequence that is highly homologous to the sequence obtained for antigen B. The predicted amino acid composition and isolectric point of paramyosin are nearly identical to those established for antigen B. Recombinant S. mansoni paramyosin, expressed in Escherichia coli as a fusion protein with beta-galactosidase, was recognized by antisera against T. solium antigen B. We conclude from these results that S. mansoni paramyosin and T. solium antigen B are homologous proteins. Since S. mansoni paramyosin is thought to be a muscle protein and T. solium antigen B a secreted glycoprotein with anti-complement activity, this conclusion raises some interesting questions regarding the role of this class of proteins in the host-parasite relationship.
抗原B是绦虫寄生虫猪带绦虫的主要抗原，已被纯化并获得了部分氨基酸序列。曼氏血吸虫的副肌球蛋白是一种免疫原性蛋白，作为疫苗候选物已显示出前景，它与抗原B具有若干生化和免疫学特性。已获得曼氏血吸虫副肌球蛋白的全长cDNA克隆，预测的翻译产物包含一个与抗原B获得的序列高度同源的序列。预测的副肌球蛋白氨基酸组成和等电点与为抗原B确定的几乎相同。在大肠杆菌中作为与β-半乳糖苷酶的融合蛋白表达的重组曼氏血吸虫副肌球蛋白被抗猪带绦虫抗原B的抗血清识别。从这些结果我们得出结论，曼氏血吸虫副肌球蛋白和猪带绦虫抗原B是同源蛋白。由于曼氏血吸虫副肌球蛋白被认为是一种肌肉蛋白，而猪带绦虫抗原B是一种具有抗补体活性的分泌糖蛋白，这一结论引发了一些关于这类蛋白在宿主-寄生虫关系中作用的有趣问题。
