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检测感染胸膜肺炎放线杆菌的猪肺组织中的 microRNAs。

Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae.

机构信息

Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Faculty of Health and Medical Sciences, Copenhagen, Denmark.

出版信息

BMC Genomics. 2012 Sep 6;13:459. doi: 10.1186/1471-2164-13-459.

DOI:10.1186/1471-2164-13-459
PMID:22953717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3465251/
Abstract

BACKGROUND

MicroRNAs (miRNAs) are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse. Actinobacillus pleuropneumoniae (APP) causes serious lung infections in pigs. Severe damage to the lungs, in many cases deadly, is caused by toxins released by the bacterium and to some degree by host mediated tissue damage. However, understanding of the role of microRNAs in the course of this infectious disease in porcine is still very limited.

RESULTS

In this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with Actinobacillus pleuropneumoniae was subjected to small RNA deep sequencing. We identified 169 conserved and 11 candidate novel microRNAs in the pig. Of these, 17 were significantly up-regulated in the necrotic sample and 12 were down-regulated. The expression analysis of a number of candidates revealed microRNAs of potential importance in the innate immune response. MiR-155, a known key player in inflammation, was found expressed in both samples. Moreover, miR-664-5p, miR-451 and miR-15a appear as very promising candidates for microRNAs involved in response to pathogen infection.

CONCLUSIONS

This is the first study revealing significant differences in composition and expression profiles of miRNAs in lungs infected with a bacterial pathogen. Our results extend annotation of microRNA in pig and provide insight into the role of a number of microRNAs in regulation of bacteria induced immune and inflammatory response in porcine lung.

摘要

背景

MicroRNAs（miRNAs）是一类非编码基因，在哺乳动物的发育和疾病中发挥着关键的调控作用。尽管近年来在结构水平上注释了大量的 miRNAs，但功能注释仍然很少。胸膜肺炎放线杆菌（APP）会导致猪的肺部严重感染。细菌释放的毒素以及在一定程度上宿主介导的组织损伤会导致肺部严重受损，在许多情况下是致命的。然而，对于 miRNA 在这种猪传染性疾病过程中的作用的理解仍然非常有限。

结果

在这项研究中，从感染胸膜肺炎放线杆菌的肉眼未见影响和坏死组织中提取 RNA，进行小 RNA 深度测序。我们在猪中鉴定了 169 个保守和 11 个候选新的 microRNAs。其中，17 个在坏死样本中显著上调，12 个下调。对一些候选物的表达分析揭示了在先天免疫反应中具有潜在重要性的 microRNAs。miR-155 是炎症的已知关键调节因子，在两个样本中均有表达。此外，miR-664-5p、miR-451 和 miR-15a 似乎是参与病原体感染反应的非常有前途的候选物。

结论

这是首次揭示细菌感染肺部的 miRNA 组成和表达谱存在显著差异的研究。我们的研究结果扩展了猪 miRNA 的注释，并深入了解了一些 miRNA 在调节猪肺部细菌诱导的免疫和炎症反应中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/a5eebd1893ed/1471-2164-13-459-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/7ca0810003d7/1471-2164-13-459-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/91b962c16642/1471-2164-13-459-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/e0337d1279d4/1471-2164-13-459-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/fe8399107f8f/1471-2164-13-459-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/a5eebd1893ed/1471-2164-13-459-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/7ca0810003d7/1471-2164-13-459-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/91b962c16642/1471-2164-13-459-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/e0337d1279d4/1471-2164-13-459-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/fe8399107f8f/1471-2164-13-459-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a13/3465251/a5eebd1893ed/1471-2164-13-459-5.jpg

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本文引用的文献

1
Ensembl 2012.Ensembl 2012.
Nucleic Acids Res. 2012 Jan;40(Database issue):D84-90. doi: 10.1093/nar/gkr991. Epub 2011 Nov 15.
2
The Reactome BioMart.Reactome 生物信息资源整合平台。
Database (Oxford). 2011 Oct 19;2011:bar031. doi: 10.1093/database/bar031. Print 2011.
3
Small RNA discovery and characterisation in eukaryotes using high-throughput approaches.真核生物中小 RNA 的高通量发现和鉴定。
循环 microRNAs 与潜伏性结核感染发展为活动性疾病风险的关联：一项巢式病例对照研究。
Microbiol Spectr. 2022 Jun 29;10(3):e0262521. doi: 10.1128/spectrum.02625-21. Epub 2022 Apr 18.
4
Comparative Mucous miRomics in Cynoglossus semilaevis Related to Vibrio harveyi Caused Infection.草鱼粘液 miRNA 组比较分析与哈维弧菌感染的关系
Mar Biotechnol (NY). 2021 Oct;23(5):766-776. doi: 10.1007/s10126-021-10062-3. Epub 2021 Sep 3.
5
Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with .人工感染[病原体名称未给出]的荷斯坦奶牛乳腺中miRNA和mRNA表达谱的综合分析
Pathogens. 2021 Apr 22;10(5):506. doi: 10.3390/pathogens10050506.
6
miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.miRNA 在农场动物疾病中的调控功能，以及有效治疗的生物标志物潜力。
Int J Mol Sci. 2021 Mar 17;22(6):3080. doi: 10.3390/ijms22063080.
7
Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs ().猪中miR-155和miR-181的表达及其结构和靶标的预测（）
Vet World. 2020 Aug;13(8):1667-1673. doi: 10.14202/vetworld.2020.1667-1673. Epub 2020 Aug 21.
8
Differentially Expressed MiRNAs and tRNA Genes Affect Host Homeostasis During Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infections in Young Pigs.差异表达的微小RNA和转运RNA基因在仔猪感染高致病性猪繁殖与呼吸综合征病毒期间影响宿主内环境稳定。
Front Genet. 2019 Aug 2;10:691. doi: 10.3389/fgene.2019.00691. eCollection 2019.
9
Global Gene Networks in 3D4/31 Porcine Alveolar Macrophages Treated with Antigenic Epitopes of Actinobacillus pleuropneumoniae ApxIA, IIA, and IVA.在经胸膜肺炎放线杆菌 ApxIA、IIA 和 IVA 抗原表位处理的 3D4/31 猪肺泡巨噬细胞中的全局基因网络。
Sci Rep. 2019 Mar 27;9(1):5269. doi: 10.1038/s41598-019-41748-3.
10
IFN-λ and microRNAs are important modulators of the pulmonary innate immune response against influenza A (H1N2) infection in pigs.干扰素-λ 和 microRNAs 是猪肺部固有免疫反应针对甲型流感（H1N2）感染的重要调节因子。
PLoS One. 2018 Apr 20;13(4):e0194765. doi: 10.1371/journal.pone.0194765. eCollection 2018.
Adv Exp Med Biol. 2011;722:239-54. doi: 10.1007/978-1-4614-0332-6_16.
4
MicroRNA-21 inhibits toll-like receptor 2 agonist-induced lung inflammation in mice.微小RNA-21抑制Toll样受体2激动剂诱导的小鼠肺部炎症。
Exp Lung Res. 2011 Oct;37(8):500-8. doi: 10.3109/01902148.2011.596895. Epub 2011 Sep 6.
5
From structure prediction to genomic screens for novel non-coding RNAs.从结构预测到新型非编码 RNA 的基因组筛选。
PLoS Comput Biol. 2011 Aug;7(8):e1002100. doi: 10.1371/journal.pcbi.1002100. Epub 2011 Aug 4.
6
MicroRNAs in development and disease.微小 RNA 在发育和疾病中的作用。
Physiol Rev. 2011 Jul;91(3):827-87. doi: 10.1152/physrev.00006.2010.
7
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers.用 DNA 引物进行 miRNA 的特异性和灵敏性定量 RT-PCR。
BMC Biotechnol. 2011 Jun 25;11:70. doi: 10.1186/1472-6750-11-70.
8
A trio of microRNAs that control Toll-like receptor signalling.调控 Toll 样受体信号通路的三个 microRNA。
Int Immunol. 2011 Jul;23(7):421-5. doi: 10.1093/intimm/dxr034. Epub 2011 Jun 6.
9
Altered expression of microRNA in the airway wall in chronic asthma: miR-126 as a potential therapeutic target.慢性哮喘气道壁中 microRNA 的表达改变：miR-126 作为潜在的治疗靶点。
BMC Pulm Med. 2011 May 23;11:29. doi: 10.1186/1471-2466-11-29.
10
MicroRNAs in NF-kappaB signaling.微小 RNA 与 NF-κB 信号通路。
J Mol Cell Biol. 2011 Jun;3(3):159-66. doi: 10.1093/jmcb/mjr007. Epub 2011 Apr 18.