可可多酚可被肠道上皮的 Caco-2 细胞模型吸收。

Cocoa polyphenols are absorbed in Caco-2 cell model of intestinal epithelium.

机构信息

University of Applied Sciences Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion, Switzerland.

出版信息

Food Chem. 2012 Dec 1;135(3):999-1005. doi: 10.1016/j.foodchem.2012.05.101. Epub 2012 Jun 7.

DOI:10.1016/j.foodchem.2012.05.101

Abstract

Cocoa is an abundant source of polyphenols, mainly flavan-3-ol monomers and polymers. In the literature, there are contradictory data on the absorption limit of procyanidins in humans. In our study, the Caco-2 cell model of intestinal epithelium was used to determine the absorption and secretion of cocoa flavan-3-ols. Three compounds: (+)-catechin, (-)-epicatechin and procyanidin B2 were detected and quantified at the receiver side of Caco-2 monolayer after 2h transport experiment. The obtained results of apparent permeability coefficient suggest paracellular route of transport of investigated compounds. Additionally, the results suggest that compounds of cocoa powder purified extract are able to affect tight junction functioning.

摘要

可可豆是多酚的丰富来源，主要是黄烷-3-醇单体和聚合物。在文献中，关于原花青素在人体内的吸收极限存在矛盾的数据。在我们的研究中，使用 Caco-2 肠上皮细胞模型来确定可可黄烷-3-醇的吸收和分泌。在 2 小时的运输实验后，在 Caco-2 单层的接收侧检测和定量了三种化合物：（+）-儿茶素、（-）-表儿茶素和原花青素 B2。表观渗透系数的结果表明，所研究的化合物是通过细胞旁途径运输的。此外，结果表明，可可粉纯化提取物中的化合物能够影响紧密连接的功能。