Departamento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.
Food Chem. 2012 Dec 1;135(3):1044-51. doi: 10.1016/j.foodchem.2012.05.089. Epub 2012 May 30.
The aim of the present study was to evaluate the vascular effects of an enzymatic extract of grape pomace (GP-EE) on isolated arteries, focusing our attention on endothelium-derived relaxation and on its antioxidant properties. Grape pomace derived from wine making was extracted by an enzymatic process and its composition of polyphenols was evaluated by HPLC and ESI-MS/MS, detecting kaempferol, catechin, quercetin and procyanidins B1 and B2, trace levels of resveratrol and tracing out gallocatechin and anthocyanidins. GP-EE induced endothelium- and NO-dependent vasodilatation of both rat aorta and small mesenteric artery (SMA) segments and reduced Phe-induced response in aortic rings. Both ORAC and DPPH assays confirmed antioxidant scavenging properties of GP-EE, which also prevented O(2)(·-) production (assessed by DHE fluorescence) and contraction elicited by ET-1. These results provide evidence that GP-EE possesses interesting antioxidant and protective vascular properties and highlight the potential interest of this extract as a functional food.
本研究旨在评估葡萄渣酶解提取物（GP-EE）对离体动脉的血管作用，重点关注内皮衍生的舒张作用及其抗氧化特性。采用酶法从酿酒葡萄渣中提取，采用 HPLC 和 ESI-MS/MS 评价其多酚组成，检测到山柰酚、儿茶素、槲皮素、原花青素 B1 和 B2、微量白藜芦醇，并追踪出没食子儿茶素和花色苷。GP-EE 诱导大鼠主动脉和小肠系膜动脉（SMA）段内皮和一氧化氮（NO）依赖性血管舒张，并降低 Phe 诱导的主动脉环反应。ORAC 和 DPPH 测定均证实了 GP-EE 的抗氧化清除特性，其还可预防 DHE 荧光评估的 O(2)(·-)产生和 ET-1 引起的收缩。这些结果表明，GP-EE 具有有趣的抗氧化和保护血管特性，并强调了这种提取物作为功能性食品的潜在兴趣。
