Plant Products and Human Nutrition Group, Joseph Black Building, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Food Chem. 2012 Dec 1;135(3):1863-7. doi: 10.1016/j.foodchem.2012.05.108. Epub 2012 Jun 7.
HPLC with fluorescence, PDA and mass spectrometric detection were used to analyse the (poly)phenol content of a Bordeaux red wine, POM Wonderful pomegranate juice and three other beverages advertised as being made from 100% pomegranate juice. The red wine and POM Wonderful juice contained characteristic anthocyanin profiles with the latter also being characterised by the presence of ellagitannins and the former by flavan-3-ols monomers and procyanidin dimers and trimers. The three other pomegranate products all contained the expected ellagitannins but their anthocyanin profiles were a mixture of red wine and pomegranate anthocyanins. They also contained flavan-3-ol monomers and procyanidin dimers and trimers, components not usually detected in 100% pomegranate beverages. The HPLC-based procedures, therefore, provide a straight-forward means of accessing the authenticity of pomegranate-based products with the ready detection of constituents derived from red grapes.
采用高效液相色谱法（HPLC）结合荧光、光电二极管阵列（PDA）和质谱检测技术，对波尔多红葡萄酒、POM 奇妙石榴汁和其他三种号称 100%石榴汁的饮料中的（多）酚类物质含量进行了分析。红葡萄酒和 POM 奇妙石榴汁中含有特征性的花色苷图谱，后者还含有鞣花单宁，而前者则含有黄烷-3-醇单体、原花青素二聚体和三聚体。其他三种石榴产品均含有预期的鞣花单宁，但它们的花色苷图谱是红葡萄酒和石榴花色苷的混合物。它们还含有黄烷-3-醇单体和原花青素二聚体和三聚体，这些成分通常不会在 100%石榴饮料中检测到。因此，基于 HPLC 的方法为石榴产品的真实性提供了一种直接的检测手段，可快速检测到来自红葡萄的成分。
