[大鼠心脏的阴离子敏感性核ATP酶]
[Anion-sensitive nuclear ATPase of the rat heart].
作者信息
Ivashchenko A T
出版信息
Vopr Med Khim. 1977 Sep-Oct;23(5):629-32.
Properties of HCO--3-stimulated ATPase from rat heart muscle nuclei were studied. The maximal activity of HCO--3-ATPase was observed at concentration of bicarbonate 25 mM. The enzyme had a pH optimum at pH 8.0-8.5. Bicarbonate stimulated the ATPase activity only in presence of Mg2+, Mn2+ and Zn2+, Co2+, Cd2+ and Ca2+ were ineffective. NaCO3 and Na2SO3 at concentration 30 mM stimulated the nuclear ATPase activity by 20% and 81%, respectively. Anions N3--, scn--, clO--4, and I-- inhibited both Mg2+-ATPase and HCO--3-ATPase. HSO--3 and SO2--4 ions did not affect the nuclear ATPase activity.
对来自大鼠心肌细胞核的HCO₃⁻刺激的ATP酶的特性进行了研究。在碳酸氢盐浓度为25 mM时观察到HCO₃⁻ - ATP酶的最大活性。该酶在pH 8.0 - 8.5时有最佳pH值。碳酸氢盐仅在Mg²⁺、Mn²⁺和Zn²⁺存在时刺激ATP酶活性，Co²⁺、Cd²⁺和Ca²⁺无效。浓度为30 mM的Na₂CO₃和Na₂SO₃分别使细胞核ATP酶活性提高了20%和81%。阴离子N₃⁻、SCN⁻、ClO₄⁻和I⁻抑制Mg²⁺ - ATP酶和HCO₃⁻ - ATP酶。HSO₃⁻和SO₄²⁻离子不影响细胞核ATP酶活性。
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