Ivashchenko A T, Li T, Bushneva I A
Vopr Med Khim. 1985 Jan-Feb;31(1):117-21.
Properties of the anion ATPase from rat red blood cell membranes were investigated. Diethyl ether treated membranes exhibited the increased activity of the anion ATPase. Na+, K+- and Ca2+-ATPase activities were not found in these preparations. The pH optimum of the anion ATPase was at pH 8.5. The enzyme was stimulated by methanol and inhibited by glycerol. Among the inorganic anions stimulators, inhibitors and indifferent substances were observed. Anions of thiocyanate, sulfite and bicarbonate altered noncompetitively the ATPase activity. Sulfite stimulated and thiocyanate inhibited the ATP hydrolysis in presence of magnesium, calcium, zinc, cobalt, manganese and nickel. Reactions with ATP, ITP, GTP but not with ADP and AMP used as substrates were sensitive to sulfite and thiocyanate. EGTA did not change the stimulation and inhibition effects of the anions on the ATPase activity. The similarity of properties of erythrocyte and mitochondrial ATPases is discussed.
对大鼠红细胞膜阴离子ATP酶的特性进行了研究。经乙醚处理的膜显示出阴离子ATP酶活性增加。在这些制剂中未发现Na +、K +和Ca2 + -ATP酶活性。阴离子ATP酶的最适pH值为8.5。该酶受甲醇刺激，受甘油抑制。在无机阴离子中观察到了刺激剂、抑制剂和无影响物质。硫氰酸盐、亚硫酸盐和碳酸氢盐的阴离子非竞争性地改变了ATP酶活性。在镁、钙、锌、钴、锰和镍存在的情况下，亚硫酸盐刺激而硫氰酸盐抑制ATP水解。以ATP、ITP、GTP而非ADP和AMP作为底物的反应对亚硫酸盐和硫氰酸盐敏感。乙二醇双四乙酸（EGTA）不改变阴离子对ATP酶活性的刺激和抑制作用。讨论了红细胞和线粒体ATP酶特性的相似性。
