Graduate School, University of Santo Tomas, España, Manila, Philippines.
J Ethnopharmacol. 2012 Oct 31;144(1):217-9. doi: 10.1016/j.jep.2012.08.019. Epub 2012 Aug 27.
Antidesma bunius Spreng. (Phyllantaceae), Averrhoa bilimbi L. (Oxalidaceae), Biophytum sensitivum (L.) DC. (Oxalidaceae), Ceriops tagal (Perr.) C.B. Rob. (Rhizophoraceae), Kyllinga monocephala Rottb. (Cyperaceae), and Rhizophora mucronata Lam. (Rhizophoraceae) are used as remedies to control diabetes. In the present study, these plants were screened for their potential α-glucosidase inhibitory activity.
The 80% aqueous ethanolic extracts were screened for their α-glucosidase enzyme inhibitory activity using yeast alpha glucosidase enzyme.
Except for A. bilimbi with IC(50) at 519.86±3.07, all manifested a significant enzyme inhibitory activity. R. mucronata manifested the highest activity with IC(50) at 0.08±1.82 μg mL(-1), followed by C. tagal with IC(50) at 0.85±1.46 μg mL(-1) and B. sensitivum with IC(50) at 2.24±1.58 μg mL(-1).
This is the first report on the α-glucosidase inhibitory effect of the six Philippine plants; thus, partly defining the mechanism on why these medicinal plants possess antidiabetic properties.
Antidesma bunius Spreng.（茜草科）、Averrhoa bilimbi L.（酢浆草科）、Biophytum sensitivum（L.）DC.（酢浆草科）、Ceriops tagal（Perr.）C.B. Rob.（红树科）、Kyllinga monocephala Rottb.（莎草科）和 Rhizophora mucronata Lam.（红树科）被用作治疗糖尿病的药物。在本研究中，这些植物被筛选以评估其潜在的α-葡萄糖苷酶抑制活性。
采用酵母α-葡萄糖苷酶，对 80%的水醇提取物进行α-葡萄糖苷酶抑制活性筛选。
除了 A. bilimbi 的 IC₅₀值为 519.86±3.07μg/mL 外，其他植物均表现出显著的酶抑制活性。R. mucronata 的活性最高，IC₅₀值为 0.08±1.82μg/mL，其次是 C. tagal 的 IC₅₀值为 0.85±1.46μg/mL 和 B. sensitivum 的 IC₅₀值为 2.24±1.58μg/mL。
这是首次报道六种菲律宾植物对α-葡萄糖苷酶的抑制作用，部分解释了这些药用植物具有降血糖特性的机制。
