Muñoz Maria Nilda M, Alvarado Urdujah G, Reyes Jerica Isabel L, Watanabe Kozo
Research & Development Extension, Cagayan State University, Tuguegarao City, Philippines.
Center for Natural Sciences and Environmental Research (CENSER), De La Salle University, Metro Manila, Philippines.
Toxicol Rep. 2021 Jun 17;8:1289-1299. doi: 10.1016/j.toxrep.2021.06.010. eCollection 2021.
The aim of the study is to assess the acute oral toxicity of ethanolic extracts of (L.) Spreng [Bignay] in ICR mice in accordance to OECD guideline 423. Single doses of Bignay fruit extracts ranging from 500 mg/kg to 2000 mg/kg, as well as a vehicle control, were given orally and monitored for 14 days. We observed that there is no mortality or adverse effects after treatment of mice indicating that Bignay extracts are safe for use in laboratory animals. Behavioral, respiratory, and neurologic changes, as well as changes in body weight, food and water consumption, did not occur during the experimental period. Hematological- (total-red and -white blood cells) and biochemical-profile analysis (alanine transaminase, blood urea nitrogen, and creatinine) remained within normal concentrations in treated mice regardless of sex. The morphology of visceral organs in all treated mice was typical in appearance when stained with H&E. It is assumed that the LD is greater than 2000 mg/kg and there is no mortality at the maximum dose used (2000 mg/kg). We have also determined the active components of dried Bignay by headspace GC-MS and by phytochemical analysis of ethanolic Bignay extracts. We have identified 16 compounds by GC-MS and 10 secondary metabolites. In this study, the assessment of extracted (L.) Spreng fruits [Bignay] on the value of safety measures in mice is described.
本研究的目的是根据经合组织准则423评估锡叶藤（L.）Spreng[野牡丹科酸脚杆属植物]乙醇提取物对ICR小鼠的急性经口毒性。将500mg/kg至2000mg/kg的单剂量野牡丹科酸脚杆属植物果实提取物以及溶剂对照组经口给予小鼠，并监测14天。我们观察到，处理小鼠后未出现死亡或不良反应，这表明野牡丹科酸脚杆属植物提取物在实验动物中使用是安全的。在实验期间，未出现行为、呼吸和神经方面的变化，以及体重、食物和水消耗量的变化。无论性别如何，处理小鼠的血液学指标（总红细胞和白细胞）和生化指标分析（丙氨酸转氨酶、血尿素氮和肌酐）均保持在正常浓度范围内。用苏木精和伊红染色后，所有处理小鼠的内脏器官形态外观均正常。据推测，半数致死剂量大于2000mg/kg，且在所用最大剂量（2000mg/kg）下未出现死亡。我们还通过顶空气相色谱-质谱联用仪以及对野牡丹科酸脚杆属植物乙醇提取物进行植物化学分析，确定了干燥野牡丹科酸脚杆属植物的活性成分。我们通过气相色谱-质谱联用仪鉴定出16种化合物和10种次生代谢产物。在本研究中，描述了对提取的锡叶藤（L.）Spreng果实[野牡丹科酸脚杆属植物]在小鼠安全措施价值方面的评估。
