Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Int J Gynecol Cancer. 2012 Oct;22(8):1297-302. doi: 10.1097/IGC.0b013e318265d334.
This study aimed to investigate the association of stromal cell-derived factor 1 (SDF-1) gene polymorphisms with the neoplastic lesions of uterine cervix in Mid-Taiwan women.
Four hundred ninety-eight blood samples were collected from 161 patients with neoplasia of uterine cervix, including 76 cancer patients, 61 patients with high-grade dysplasia, and 24 with low-grade dysplasia, and 337 healthy controls who lived in Mid-Taiwan. Polymorphism of the SDF-1 gene was examined using polymerase chain reaction-restriction fragment length polymorphism.
For SDF-1 gene polymorphisms, the wild-type homozygous alleles (G/G) yielded 100- and 193-bp products, the heterozygous alleles (G/A) yielded 100-, 193- and 293-bp products, whereas the mutated-type homozygous alleles (A/A) yielded a 293-bp product. We found no significant difference in genotypes or alleles distribution of SDF-1 polymorphisms between patients with cervical neoplasia and healthy women (P = 0.530). Compared with the homozygous GG subgroup, GA and AA subgroups do not increase the risk of cervical neoplasia.
Although the expression of SDF-1 was reported to be significantly increased in cervical carcinogenesis in previous studies, our results, however, show that SDF-1 gene polymorphism could not be considered as a factor related to an increased susceptibility to cervical neoplasia.
本研究旨在探讨基质细胞衍生因子 1（SDF-1）基因多态性与台湾中南部女性宫颈肿瘤病变的关系。
从 161 例患有宫颈肿瘤的患者中采集了 498 份血液样本，包括 76 例癌症患者、61 例高级别发育不良患者和 24 例低级别发育不良患者，以及 337 名居住在台湾中南部的健康对照者。使用聚合酶链反应-限制性片段长度多态性检测 SDF-1 基因的多态性。
对于 SDF-1 基因多态性，野生型纯合子等位基因（G/G）产生 100-和 193-bp 产物，杂合子等位基因（G/A）产生 100-、193-和 293-bp 产物，而突变型纯合子等位基因（A/A）产生 293-bp 产物。我们发现宫颈肿瘤患者和健康女性之间 SDF-1 多态性的基因型或等位基因分布没有显著差异（P = 0.530）。与纯合子 GG 亚组相比，GA 和 AA 亚组不会增加宫颈癌的风险。
尽管之前的研究报道 SDF-1 的表达在宫颈癌变过程中显著增加，但我们的结果表明，SDF-1 基因多态性不能被认为是与宫颈癌易感性增加相关的因素。
