Department of Clinical & Chemical Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
Department of Internal Medicine, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
Egypt J Immunol. 2021 Jul 1;28(3):94-101.
Stromal cell derived factor - 1 (SDF-1) is expressed by bone marrow derived stromal cells and stromal tissues in multiple organs. SDF-1 possesses diverse physiological and biochemical functions concerning autoimmunity and inflammation. SDF-1 gene polymorphisms play various roles in immune related diseases. This study aimed to evaluate association of SDF-1β G801A gene polymorphism and type 2 diabetes mellitus (T2DM) as regard prevalence and glycemic control. The study included hundred Egyptian subjects; 50 patients with T2DM recruited from the Internal Medicine Department, Beni-Suef University Hospital, and 50 age and sex matched healthy subjects. Participants were subjected to full clinical examination and routine laboratory investigations including fasting blood glucose, 2 hours post prandial blood glucose, serum creatinine, lipid profile and glycated hemoglobin (HbA1c). SDF-1β G801A gene polymorphism was analyzed using polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP). The frequency of GG and AG genotypes was higher in patients compared to controls with no statistical significance. Higher frequency of homozygous GG genotype and G allele were observed among patients with poor glycemic control (HbA1c ≥ 8% ) compared to those with good glycemic control (HbA1c < 8%) (P = 0.001 and 0.018 respectively). In conclusion, this study suggested an association between SDF-1 β gene polymorphism and type 2 diabetes mellitus in Egyptian patients. Further studies are needed to confirm our observations.
基质细胞衍生因子-1（SDF-1）由骨髓来源的基质细胞和多个器官的基质组织表达。SDF-1 具有与自身免疫和炎症有关的多种生理和生化功能。SDF-1 基因多态性在免疫相关疾病中发挥着不同的作用。本研究旨在评估 SDF-1β G801A 基因多态性与 2 型糖尿病（T2DM）之间的关联，包括患病率和血糖控制。该研究纳入了 100 名埃及受试者；50 名 T2DM 患者来自本尼苏夫大学医院内科，50 名年龄和性别匹配的健康受试者。参与者接受了全面的临床检查和常规实验室检查，包括空腹血糖、餐后 2 小时血糖、血清肌酐、血脂谱和糖化血红蛋白（HbA1c）。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 SDF-1β G801A 基因多态性。与对照组相比，患者的 GG 和 AG 基因型频率更高，但无统计学意义。在血糖控制不佳（HbA1c≥8%）的患者中，纯合 GG 基因型和 G 等位基因的频率高于血糖控制良好（HbA1c<8%）的患者（分别为 P=0.001 和 0.018）。总之，本研究提示 SDF-1β 基因多态性与埃及患者的 2 型糖尿病之间存在关联。需要进一步的研究来证实我们的观察结果。
