Faa G, Gerosa C, Fanni D, Nemolato S, Di Felice E, Van Eyken P, Monga G, Iacovidou N, Fanos V
Department of Pathology, University of Cagliari, Cagliari, Italy.
J Matern Fetal Neonatal Med. 2012 Oct;25 Suppl 4:135-8. doi: 10.3109/14767058.2012.715018.
The identification of the different cell types involved in human nephrogenesis, when solely based on morphology, may lead to errors in its interpretation, given the complexity of the histological picture of the fetal and of the newborn kidney. In this study, the most recent works utilizing immunohistochemistry for the identification of the multiple cell types involved in human nephrogenesis are reviewed. The role of WT1, MUC1, Thymosin beta 10, Thymosin beta 4, CD10 and CD44 in the different phases of glomerulogenesis and of tubulogenesis is here described, with particular emphasis on their expression in the early phases of nephrogenesis. On the basis of our data, immunohistochemistry appears to be a useful tool in the study of human nephrogenesis, giving new data on the different steps of the differentiation of metanephric mesenchyme towards the multiple cell types characterizing the mature human kidney. Moreover, allowing a better knowledge of the protein products involved in the generation of new nephrons, immunohistochemistry could open new perspectives in the field of renal regenerating medicine, evidencing the factors able to prolong nephrogenesis after birth, helping us to reach our goal: allowing newborn kidneys to restore their nephron endowment, escaping susceptibility to hypertension and renal disease in adulthood.
鉴于胎儿和新生儿肾脏组织学图像的复杂性，仅基于形态学来识别参与人类肾发生的不同细胞类型，可能会导致其解释出现错误。在本研究中，我们回顾了利用免疫组织化学来识别参与人类肾发生的多种细胞类型的最新研究成果。本文描述了WT1、MUC1、胸腺素β10、胸腺素β4、CD10和CD44在肾小球发生和肾小管发生不同阶段的作用，特别强调了它们在肾发生早期阶段的表达。根据我们的数据，免疫组织化学似乎是研究人类肾发生的一个有用工具，它为后肾间充质向构成成熟人类肾脏的多种细胞类型分化的不同步骤提供了新的数据。此外，免疫组织化学能够让我们更好地了解参与新肾单位生成的蛋白质产物，从而在肾脏再生医学领域开辟新的前景，揭示能够延长出生后肾发生的因素，帮助我们实现目标：让新生儿肾脏恢复其肾单位数量，避免成年后患高血压和肾脏疾病。
