Gerosa Clara, Fanni Daniela, Nemolato Sonia, Locci Annalisa, Marinelli Viviana, Cabras Tiziana, Messana Irene, Castagnola Massimo, Monga Guido, Fanos Vassilios, Faa Gavino
Department of Pathology, University of Cagliari, Cagliari, Italy.
J Matern Fetal Neonatal Med. 2010 Oct;23 Suppl 3:125-8. doi: 10.3109/14767058.2010.510645.
Thymosin beta-10 (Tβ10) is a member of beta-thymosins (Tβs), a family of low molecular mass peptides, which play essential roles in many cellular functions, including apoptosis, cell proliferation, cell migration, and endocytosis. The report that the Tβ10 gene is expressed at high levels in embryonic human brain as well in human kidney induced us to study Tβ10 reactivity in the preterm kidney in order to verify, at tissue level, the expression of this peptide during renal embryogenesis. To this end, we analyzed, using immunocytochemistry, the expression of Tβ10 in samples of human kidney obtained, at autopsy, from 8 fetuses, 12 preterm infants, ranging from 25 to 36 weeks of gestation and 3 at term newborns. Tβ10 immunoreactivity was detected in 20 out of 22 kidneys examined, and was mainly localized in proximal and distal tubular structures, in the cytoplasm and occasionally in the nuclei of ductal cells. In 11 cases, we also detected a focal and mild reactivity for the peptide in glomeruli. In 13 kidneys, we also observed immunostaining for Tβ10 inside the "comma-shaped bodies" and the "S-shaped bodies" during active glomerulogenesis. Our data show, for the first time, the expression of Tβ10 in the human kidney during the initial phases of its physiological development, mainly restricted in the proximal and the distal tubuli. Further studies are needed in order to better characterize the role of Tβ10 in kidney embryogenesis.
胸腺素β-10（Tβ10）是β-胸腺素（Tβs）家族的成员之一，β-胸腺素是一类低分子量肽，在许多细胞功能中发挥着重要作用，包括细胞凋亡、细胞增殖、细胞迁移和内吞作用。有报道称Tβ10基因在人类胚胎脑以及人类肾脏中高水平表达，这促使我们研究早产肾脏中Tβ10的反应性，以便在组织水平验证该肽在肾脏胚胎发生过程中的表达。为此，我们采用免疫细胞化学方法分析了从8例胎儿、12例孕周为25至36周的早产儿以及3例足月新生儿尸检获取的人类肾脏样本中Tβ10的表达。在所检查的22个肾脏中，有20个检测到了Tβ10免疫反应性，其主要定位于近端和远端肾小管结构、细胞质中，偶尔也定位于导管细胞的细胞核中。在11例病例中，我们还在肾小球中检测到了该肽的局灶性轻度反应性。在13个肾脏中，我们还观察到在活跃的肾小球发生过程中，“逗号状小体”和“S形小体”内部有Tβ10免疫染色。我们的数据首次显示了Tβ10在人类肾脏生理发育初始阶段的表达，主要局限于近端和远端小管。为了更好地描述Tβ10在肾脏胚胎发生中的作用，还需要进一步研究。
