Analytical and Testing Center, Shanxi Normal University, Linfen, Shanxi 041004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Dec;98:275-81. doi: 10.1016/j.saa.2012.08.016. Epub 2012 Aug 19.
Amantadine hydrochloride (AMA) and rimantadine hydrochloride (RIM) are non-fluorescent in aqueous solutions. This property makes their determination through direct fluorescent method difficult. The competing reactions and the supramolecular interaction mechanisms between the two drugs and coptisine (COP) as they fight for occupancy of the cucurbit[7]uril (CB[7]) cavity, were studied using spectrofluorimetry, (1)H NMR, and molecular modeling calculations. Based on the significant quenching of the supramolecular complex fluorescence intensity, a fluorescent probe method of high sensitivity and selectivity was developed to determine AMA or RIM in their pharmaceutical dosage forms and in urine samples with good precision and accuracy. The linear range of the method was from 0.0040 to 1.0 μg mL(-1) with a detection limit ranging from 0.0012 to 0.0013 μg mL(-1). This shows that the proposed method has promising potential for therapeutic monitoring and pharmacokinetics and for clinical application.
盐酸金刚烷胺（AMA）和盐酸金刚烷乙胺（RIM）在水溶液中无荧光。这一特性使得通过直接荧光法来测定它们变得困难。本文使用荧光光谱法、（1）H NMR 和分子建模计算，研究了两种药物与小檗碱（COP）之间的竞争反应和超分子相互作用机制，以及它们为占据瓜环（CB[7]）空腔而进行的竞争。基于超分子配合物荧光强度的显著猝灭，建立了一种高灵敏度和选择性的荧光探针法，用于测定其药物制剂和尿液样品中的 AMA 或 RIM，具有良好的精密度和准确度。该方法的线性范围为 0.0040 至 1.0 μg mL(-1)，检测限范围为 0.0012 至 0.0013 μg mL(-1)。这表明该方法在治疗监测、药代动力学和临床应用方面具有广阔的应用前景。
