利用嗜热微生物合成抗 HCV 化合物。

Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes. Roque Saenz Peña 352, Bernal (B1868BXD), Argentina.

Bioorg Med Chem Lett. 2012 Oct 1;22(19):6059-62. doi: 10.1016/j.bmcl.2012.08.045. Epub 2012 Aug 21.

This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2'-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379mg/L of 6-chloropurine-2'-deoxyriboside. The obtained compounds can be used as antiviral agents.

本工作描述了嗜热微生物在获得 6-卤代嘌呤核苷中的应用。通过嗜热脂肪地芽孢杆菌 CECT 43 实现了 6-氯嘌呤-2'-脱氧核苷和 6-氯嘌呤核苷的生物合成，转化率分别为 90%和 68%。此外，通过琼脂糖基质固定化，所选微生物得到了令人满意的稳定化。这种生物催化剂至少可以重复使用 70 次而不会显著损失活性，可获得 379mg/L 的 6-氯嘌呤-2'-脱氧核苷。所得到的化合物可用作抗病毒剂。