Biomedical Engineering Program, COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Acad Radiol. 2013 Jan;20(1):90-8. doi: 10.1016/j.acra.2012.07.013. Epub 2012 Sep 5.
The gold-standard tool for colorectal cancer detection is colonoscopy, but it provides only mucosal surface visualization. Ultrasound biomicroscopy allows a clear delineation of the epithelium and adjacent colonic layers. The aim of this study was to design a system to generate endoluminal ultrasound biomicroscopic images of the mouse colon, in vivo, in an animal model of inflammation-associated colon cancer.
Thirteen mice (Mus musculus) were used. A 40-MHz miniprobe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope. Control mice (n = 3) and mice treated with azoxymethane and dextran sulfate sodium (n = 10) were subjected to simultaneous endoluminal ultrasound biomicroscopy and white-light colonoscopy. The diagnosis obtained with endoluminal ultrasound biomicroscopy and colonoscopy was compared and confirmed by postmortem histopathology.
Endoluminal ultrasound biomicroscopic images showed all layers of the normal colon and revealed lesions such as lymphoid hyperplasias and colon tumors. Additionally, endoluminal ultrasound biomicroscopy was able to detect two cases of mucosa layer thickening, confirmed by histology. Compared to histologic results, the sensitivities of endoluminal ultrasound biomicroscopy and colonoscopy were 0.95 and 0.83, respectively, and both methods achieved specificities of 1.0.
Endoluminal ultrasound biomicroscopy can be used, in addition to colonoscopy, as a diagnostic method for colonic lesions. Moreover, experimental endoluminal ultrasound biomicroscopy in mouse models is feasible and might be used to further develop research on the differentiation between benign and malignant colonic diseases.
结直肠癌检测的金标准工具是结肠镜检查，但它只能提供黏膜表面的可视化。超声生物显微镜可清晰显示上皮和相邻结肠层。本研究旨在设计一种系统，以便在炎症相关结直肠癌的动物模型中，活体生成小鼠结肠的腔内超声生物显微镜图像。
使用 13 只小鼠（Mus musculus）。将 40MHz 微型探头导管插入小儿纤维支气管镜的辅助通道。对照组（n=3）和给予氧化偶氮甲烷和葡聚糖硫酸钠的小鼠（n=10）同时接受腔内超声生物显微镜和白光结肠镜检查。将腔内超声生物显微镜和结肠镜检查的诊断结果进行比较，并通过死后组织病理学进行确认。
腔内超声生物显微镜图像显示了正常结肠的所有层，并显示了诸如淋巴组织增生和结肠肿瘤等病变。此外，腔内超声生物显微镜还能够检测到组织学证实的两层黏膜层增厚。与组织学结果相比，腔内超声生物显微镜和结肠镜的灵敏度分别为 0.95 和 0.83，两种方法的特异性均为 1.0。
腔内超声生物显微镜除了结肠镜检查外，还可以作为一种诊断结直肠病变的方法。此外，在小鼠模型中进行实验性腔内超声生物显微镜是可行的，并且可能用于进一步开发对良性和恶性结肠疾病进行区分的研究。
