Biomedical Engineering Program, COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Ultrasound Med Biol. 2011 Dec;37(12):2086-95. doi: 10.1016/j.ultrasmedbio.2011.09.003. Epub 2011 Oct 26.
The present work tested the capability of ultrasound biomicroscopy (UBM), at 45 MHz, to provide cross-sectional images with appropriate resolution and contrast to detect tumors and determine their penetration depths on the colon of mice, Mus musculus (Linnaeus 1758), treated with carcinogen for colon tumor induction. B-mode images were obtained, in vitro, from each animal (13 treated and 4 untreated) colon opened longitudinally and immersed in saline solution at room temperature. Prior to UBM inspection, all animals were also examined by colonoscopy. The layers of normal colon identified by UBM are: mucosa (hyperechoic), muscularis mucosae (hypoechoic), submucosa (hyperechoic) and muscularis externa (hypoechoic). UBM images of colon lesions presented structures corresponding to tumors (hyperechoic), lymphoid hyperplasia (hypoechoic) and polypoid tumors (hyperechoic). Additionally, tumoral lesion invasion through the colon was also identified. When compared with histopathologic analysis, all colon lesions detected by UBM were confirmed, while colonoscopic findings had two false negatives.
本研究旨在测试 45MHz 超声生物显微镜（UBM）的能力，以提供具有适当分辨率和对比度的横截面图像，从而检测用致癌剂处理的用于结肠肿瘤诱导的小鼠（Mus musculus [Linnaeus 1758]）结肠中的肿瘤，并确定其穿透深度。对每个动物（13 个处理组和 4 个未处理组）的结肠进行体外检测，将其纵向剖开并浸泡在室温下的盐水中。在进行 UBM 检查之前，还对所有动物进行了结肠镜检查。UBM 识别的正常结肠层包括：黏膜（高回声）、黏膜肌层（低回声）、黏膜下层（高回声）和外肌层（低回声）。结肠病变的 UBM 图像显示结构对应于肿瘤（高回声）、淋巴组织增生（低回声）和息肉样肿瘤（高回声）。此外，还识别了肿瘤病变对结肠的穿透。与组织病理学分析相比，UBM 检测到的所有结肠病变均得到证实，而结肠镜检查有两个假阴性。
