Cardiovascular Division, Health Research Institute, University Clinical Hospital of Santiago de Compostela, Spain.
Atherosclerosis. 2012 Oct;224(2):492-9. doi: 10.1016/j.atherosclerosis.2012.07.014. Epub 2012 Aug 27.
Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the physiopathology of several metabolic and cardiovascular diseases, especially coronary artery disease (CAD). We aimed to study whether there are differences in the proteome and the secretome between epicardial and subcutaneous adipose tissue (SAT) from patients with and without CAD.
EAT and SAT samples were collected from 64 patients undergoing elective cardiac surgery either for coronary artery bypass grafting or valve surgery. One or two-dimensional electrophoresis were performed on tissue samples and media collected at 3, 6, 24 or 48 of tissue culture. Protein identification was performed with mass spectrometry, and the results were then validated with Western blot or enzyme immunoassay. mRNA expression levels were analysed by real time polymerase chain reaction.
The release of several proteins was found to be higher in EAT that in SAT. Remarkably, there were higher levels of apolipoprotein A-I and glutation S-transferase P release, whereas mRNA expression of fatty acid binding protein 4 was lower in EAT. Although apolipoprotein A-I protein quantity in EAT was similar between CAD and non CAD patients, its released levels from this fat pad were lower in CAD.
EAT and SAT show different profiles of protein release and a different pattern was also found in samples from patients with CAD. These findings might support the hypothesis that EAT plays an interesting role in the physiopathology of atherosclerosis and CAD.
最近的研究集中在心脏外膜脂肪组织（EAT）在几种代谢和心血管疾病，尤其是冠状动脉疾病（CAD）的病理生理学中的潜在作用。我们旨在研究 EAT 和心脏外膜下脂肪组织（SAT）之间的蛋白质组和分泌组是否存在差异，这些差异存在于 CAD 患者和非 CAD 患者之间。
从 64 名接受择期心脏手术的患者中采集 EAT 和 SAT 样本，这些患者进行冠状动脉旁路移植术或瓣膜手术。对组织样本和培养 3、6、24 或 48 小时后的介质进行一维或二维电泳。使用质谱法进行蛋白质鉴定，然后使用 Western blot 或酶联免疫吸附法进行验证。通过实时聚合酶链反应分析 mRNA 表达水平。
发现 EAT 的几种蛋白质释放量高于 SAT。值得注意的是，载脂蛋白 A-I 和谷胱甘肽 S-转移酶 P 的释放水平较高，而 EAT 中的脂肪酸结合蛋白 4 的 mRNA 表达水平较低。尽管 EAT 中的载脂蛋白 A-I 蛋白数量在 CAD 和非 CAD 患者之间相似，但从这个脂肪垫中释放的水平在 CAD 中较低。
EAT 和 SAT 显示出不同的蛋白质释放谱，在 CAD 患者的样本中也发现了不同的模式。这些发现可能支持 EAT 在动脉粥样硬化和 CAD 的病理生理学中发挥有趣作用的假说。
