长非编码 RNA 在转录基因沉默中的作用。

The role of long non-coding RNA in transcriptional gene silencing.

机构信息

University of Michigan, Department of Molecular, Cellular and Developmental Biology, Ann Arbor, MI 48109, USA.

出版信息

Curr Opin Plant Biol. 2012 Nov;15(5):517-22. doi: 10.1016/j.pbi.2012.08.008. Epub 2012 Sep 6.

DOI:10.1016/j.pbi.2012.08.008

Abstract

Transcriptional gene silencing controls the activity of transposable elements and expression of protein-coding genes. It requires non-coding transcription, which in plants is performed by RNA Polymerases IV and V (Pol IV and Pol V). Pol IV produces precursors for siRNA biogenesis while Pol V produces scaffold transcripts required for siRNAs and associated proteins to recognize their target loci. In this review I discuss the mechanisms used by Pol IV and Pol V to mediate repressive chromatin modifications. I further discuss the mechanisms controlling non-coding transcription and their role in regulation of genome activity.

摘要

转录基因沉默控制转座元件的活性和蛋白质编码基因的表达。它需要非编码转录，在植物中由 RNA 聚合酶 IV 和 V（Pol IV 和 Pol V）完成。Pol IV 产生 siRNA 生物发生的前体，而 Pol V 产生 siRNA 和相关蛋白识别其靶标所需的支架转录本。在这篇综述中，我讨论了 Pol IV 和 Pol V 用于介导抑制性染色质修饰的机制。我进一步讨论了控制非编码转录的机制及其在调节基因组活性中的作用。