评价通用 RT-nested-PCR 在巴西里约热内卢疑似登革热感染致死病例中检测黄病毒的效果。

Evaluation of a generic RT-nested-PCR for detection of flaviviruses in suspected fatal cases of dengue infection, Rio de Janeiro, Brazil.

机构信息

Laboratory of Molecular Biology for Infectious Diseases and Cancer, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, CEP 59072-910, Brazil.

出版信息

J Virol Methods. 2012 Dec;186(1-2):167-70. doi: 10.1016/j.jviromet.2012.08.010. Epub 2012 Aug 30.

DOI:10.1016/j.jviromet.2012.08.010

PMID:22960088

Abstract

Flaviviruses are significant causes of disease worldwide and can be classified serologically into several antigenic complexes. The purpose of the present study was to evaluate the effectiveness of a generic RT-nested-PCR for detection of flavivirus during a dengue outbreak in Brazil in 2008. A total of 105 serum samples were collected from patients with fatal outcome and examined by generic RT-PCR, conventional RT-PCR, and IgM serology. The generic RT-PCR confirmed 19 of 105 (18%) cases. Conventional RT-PCR performed on 105 serum samples detected 45 (42.8%) dengue virus infections. The IgM serology confirmed 44 of 102 (43.1%) cases. The infecting serotype was identified by generic RT-PCR in 19 cases (18 DENV-2 and 1 DENV-3) and by conventional RT-PCR in 45 cases (40 DENV-2 and 5 DENV-3). In addition, we analyzed the performance of the generic and conventional RT-PCRs and IgM serology on serum samples stratified by the day of onset of symptoms. Our results indicate that different methods should be included in flavivirus surveillance programs, including virological and serological approaches.

摘要

黄病毒是全球范围内重要的疾病病原体，可根据血清学特征分为几个抗原性复合物。本研究旨在评估通用 RT-巢式 PCR 在 2008 年巴西登革热疫情中的应用效果。共采集了 105 例重症患者血清样本，采用通用 RT-PCR、常规 RT-PCR 和 IgM 血清学方法进行检测。通用 RT-PCR 确认了 105 例中的 19 例（18%）。对 105 例血清样本进行常规 RT-PCR 检测，发现 45 例（42.8%）登革病毒感染。IgM 血清学检测确认了 102 例中的 44 例（43.1%）。通用 RT-PCR 鉴定了 19 例（18 例为 DENV-2 和 1 例为 DENV-3），常规 RT-PCR 鉴定了 45 例（40 例为 DENV-2 和 5 例为 DENV-3）。此外，我们还分析了通用和常规 RT-PCR 以及 IgM 血清学在按症状出现日分层的血清样本中的表现。我们的结果表明，应在黄病毒监测计划中纳入不同的方法，包括病毒学和血清学方法。