Institute of Biophysics, Chinese Academy Sciences, Beijing 100101, PR China.
Biochem Biophys Res Commun. 2012 Sep 28;426(3):399-403. doi: 10.1016/j.bbrc.2012.08.102. Epub 2012 Aug 30.
We designed a rotary biosensor as a damping effector, with the rotation of the F(0)F(1)-ATPase driven by Adenosine Triphosphate (ATP) synthesis being indicated by the fluorescence intensity and a damping effect force being induced by the binding of an RNA molecule to its probe on the rotary biosensor. We found that the damping effect could contribute to the resonance phenomenon and energy transfer process of our rotary biosensor in the liquid phase. This result indicates that the ability of the rotary motor to operate in the vibration harmonic mode depends on the environmental conditions and mechanism in that a few molecules of the rotary biosensor could induce all of the sensor molecules to fluoresce together. These findings contribute to the theory study of the ATPase motor and future development of biosensors for ultrasensitive detection.
我们设计了一种旋转生物传感器作为阻尼效应器，通过荧光强度指示由三磷酸腺苷 (ATP) 合成驱动的 F(0)F(1)-ATP 酶的旋转，并且通过 RNA 分子与其探针在旋转生物传感器上的结合来诱导阻尼效应力。我们发现，阻尼效应可以有助于我们的旋转生物传感器在液相中的共振现象和能量转移过程。这一结果表明，旋转电机在振动谐波模式下运行的能力取决于环境条件和机制，即几个旋转生物传感器分子可以诱导所有传感器分子一起发光。这些发现有助于 ATP 酶马达的理论研究和未来超灵敏检测生物传感器的发展。
